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CD79b antibody | HM79-11

Hamster anti Mouse CD79b

Product Type
Monoclonal Antibody
Clone
HM79-11
Isotype
IgG
Specificity
CD79b

Product Code Applications Pack Size List Price Your Price Qty
MCA1821GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP WB 0.1 mg loader
List Price Your Price
loader

Hamster anti Mouse CD79b antibody, clone HM79-11 recognizes murine CD79 beta, expressed by B cells as part of the B cell receptor complex (immunoglobulin and the CD79 alpha/beta heterodimer).

Murine CD79 beta is expressed at the cell surface of pro-B cells prior to surface immunoglobulin, and is expressed throughout B cell differentiation.

CD79 beta is a B cell specific marker, valuable for the detection of B cells at all maturation stages.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
CD79 alpha/CD79 beta heterodimer purified from WEHI-231 B cells.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Lymph node cells from immunized Armenian hamsters were fused with cells of the mouse X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood

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Description Goat F(ab')2 anti Hamster IgG:DyLight®650
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Description Goat F(ab')2 anti Hamster IgG:FITC
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Description Goat anti Hamster IgG:Biotin
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
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Description Hamster (Armenian) IgG Negative Control

References for CD79b antibody

  1. Koyama, M. et al. (1997) CD79 alpha/CD79 beta heterodimers are expressed on pro-B cell surfaces without associated mu heavy chain.
    Int Immunol. 9 (11): 1767-72.
  2. Jelínek, F. et al. (2005) Characteristics of two mouse bcr-abl-transformed cell lines. II. Pathological lesions induced in mice.
    Folia Biol (Praha). 51 (4): 93-102.
  3. Ankeny, D.P. et al. (2006) Spinal cord injury triggers systemic autoimmunity: evidence for chronic B lymphocyte activation and lupus-like autoantibody synthesis.
    J Neurochem. 99 (4): 1073-87.
  4. Lacoste-collin, L. et al. (2007) Effect of continuous irradiation with a very low dose of gamma rays on life span and the immune system in SJL mice prone to B-cell lymphoma.
    Radiat Res. 168 (6): 725-32.
  5. Luger, D. et al. (2013) Expression of the B-cell receptor component CD79a on immature myeloid cells contributes to their tumor promoting effects.
    PLoS One. 8 (10): e76115.

Synonyms
B29
RRID
AB_324664
UniProt
P15530
Entrez Gene
Cd79b
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0050853 B cell receptor signaling pathway

MCA1821GA

0312R 156965 165901

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