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v-myb/c-myb antibody | 10A3

Mouse anti v-myb/c-myb

Product Type
Monoclonal Antibody
Clone
10A3
Isotype
IgG2a
Specificity
v-myb/c-myb

Product Code Applications Pack Size List Price Your Price Qty
MCA1793
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 0.1 mg loader
List Price Your Price
loader

Mouse anti v-myb/c-myb antibody, clone 10A3 recognizes both v-myb and c-myb. Myb is a transcription factor related to Myc, and is expressed primarily in haematopoietic cells and immature gastroepithelial cells. Mouse anti v-myb/c-myb antibody, clone 10A3 does not recognise xenopus A-myb.

Target Species
Xenopus
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Human
Zebrafish
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant v-myb protein, DNA binding protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised B110x Balb/c F1 mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for v-myb/c-myb antibody

  1. Sleeman, J.P. (1993) Xenopus A-myb is expressed during early spermatogenesis.
    Oncogene. 8 (7): 1931-41.
  2. Grabher, C. et al. (2011) Zebrafish microRNA-126 determines hematopoietic cell fate through c-Myb.
    Leukemia. 25 (3): 506-14.

Western Blotting

RRID
AB_1125374
UniProt
Q08759
Entrez Gene
myb
GO Terms
GO:0003677 DNA binding
GO:0005634 nucleus
GO:0006350 transcription
GO:0030528 transcription regulator activity

MCA1793

149730

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