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Myeloperoxidase antibody | 2C7

Mouse anti Human Myeloperoxidase:RPE

Product Type
Monoclonal Antibody
Clone
2C7
Isotype
IgG1
Specificity
Myeloperoxidase

Product Code Applications Pack Size List Price Your Price Qty
MCA1757PE
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Mouse anti Human myeloperoxidase antibody, clone 2C7 recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55 kDa) and two light chains (15 kDa), the heavy chains being linked by a disulphide bond.

Mouse anti Human Myeloperoxidase antibody, clone 2C7 recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. Mouse anti Human Myeloperoxidase antibody, clone 2C7 also recognizes recombinant MPO in western blots and weakly in ELISA.

Mouse anti Human myeloperoxidase antibody, clone 2C7 may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeabilization. Mouse anti Human myeloperoxidase antibody, clone 2C7 did not recognize rat MPO by ELISA (Patry et al. 2003).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Dog
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Human myeloperoxidase
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse X63 AG8-653 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1 Neat
  1. 1 Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

Source Reference

  1. Audrain, M.A. et al. (1997) Anti-native and recombinant myeloperoxidase monoclonals and human autoantibodies.
    Clin Exp Immunol. 107 (1): 127-34.

References for Myeloperoxidase antibody

  1. Patry, Y.C. et al. (2003) Difference in antigenic determinant profiles between human and rat myeloperoxidase.
    Clin Exp Immunol. 132 (3): 505-8.
  2. Sloane, A.J. et al. (2005) Proteomic analysis of sputum from adults and children with cystic fibrosis and from control subjects.
    Am J Respir Crit Care Med. 172: 1416-26.
  3. Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
    Vet Clin Pathol. 35 (1): 55-71.
  4. Villiers, E. et al. (2006) Identification of acute myeloid leukemia in dogs using flow cytometry with myeloperoxidase, MAC387, and a canine neutrophil-specific antibody.
    Vet Clin Pathol. 35 (1): 55-71.
  5. Luo, B. et al. (2013) Immunopathology features of chronic rhinosinusitis in high-altitude dwelling Tibetans.
    Allergy Rhinol (Providence). 4: e69-76.
  6. Gelain, M.E. et al. (2014) CD44 in canine leukemia: analysis of mRNA and protein expression in peripheral blood.
    Vet Immunol Immunopathol. 159 (1-2): 91-6.
  7. Behnen, M. et al. (2014) Immobilized immune complexes induce neutrophil extracellular trap release by human neutrophil granulocytes via FcγRIIIB and Mac-1.
    J Immunol. 193 (4): 1954-65.
  8. Novacco, M. et al. (2016) Prognostic factors in canine acute leukaemias: a retrospective study.
    Vet Comp Oncol. 14 (4): 409-16.
  9. View The Latest Product References
  10. Wang, H. et al. (2016) Circulating Level of Neutrophil Extracellular Traps Is Not a Useful Biomarker for Assessing Disease Activity in Antineutrophil Cytoplasmic Antibody-Associated Vasculitis.
    PLoS One. 11 (2): e0148197.
  11. Rai, A.K. et al. (2017) Exonal switch down-regulates the expression of CD5 on blasts of acute T cell leukaemia.
    Clin Exp Immunol. 190 (3): 340-350.
  12. Hoppenbrouwers, T. et al. (2018) Neutrophil Extracellular Traps in Children With Meningococcal Sepsis.
    Pediatr Crit Care Med. 19 (6): e286-e291.
  13. Helseth, R. et al. (2019) Glucose associated NETosis in patients with ST-elevation myocardial infarction: an observational study.
    BMC Cardiovasc Disord. 19 (1): 221.
  14. Manfredi, A.A. et al. (2021) Platelet phagocytosis via PSGL1 and accumulation of microparticles in systemic sclerosis.
    Arthritis Rheumatol. Jul 19 [Epub ahead of print].
  15. de Moraes Mazetto, B. et al. (2022) Association between neutrophil extracellular traps (NETs) and thrombosis in antiphospholipid syndrome
    Thrombosis Res. 214: 132-7.
  16. Robinson, H. et al. (2022) The effect of expressive writing on wound healing: Immunohistochemistry analysis of skin tissue two weeks after punch biopsy wounding.
    J Psychosom Res. 161: 110987.
  17. Zhu, D. et al. (2024) Neutrophil activation biomarker pentraxin 3 for diagnosis and monitoring of macrophage activation syndrome occurrence in adult-onset Still's disease.
    J Autoimmun. 144: 103182.

RRID
AB_322880
UniProt
P05164
Entrez Gene
MPO
GO Terms
GO:0003682 chromatin binding
GO:0006952 defense response
GO:0005764 lysosome
GO:0004601 peroxidase activity
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0030141 stored secretory granule
GO:0055114 oxidation-reduction process
GO:0006916 anti-apoptosis
GO:0008201 heparin binding
GO:0020037 heme binding
GO:0034374 low-density lipoprotein particle remodeling
GO:0042744 hydrogen peroxide catabolic process

MCA1757PE

1015 158509

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