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WC1 antibody | CC101

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Anti Bovine WC1 Antibody, clone CC101 thumbnail image 1 Anti Bovine WC1 Antibody, clone CC101 thumbnail image 2
Anti Bovine WC1 Antibody, clone CC101 gallery image 1
Bovine peripheral blood lymphocytes stained with Mouse anti Bovine WC1 antibody, clone CC101 (MCA1655G) followed by Goat anti Mouse IgG (H/L):FITC (STAR117F).
Anti Bovine WC1 Antibody, clone CC101 gallery image 2
Published customer image:
Fitc conjugated Mouse anti Bovine WC1 antibody, clone CC101 (MCA1655F) used to assess WC1 ezpression on bovine cells by flow cytometry.
Image caption:
Representative dot plots. Gate strategy: (1) R1, lymphocyte gate used for WC1+ cells and (2) R2, gate used for NKp46+ cells. Dot plots from representative samples of WC1+IFN-γ+ in line A and NKp46+ IFN-γ+ in line B for non-infected and infected co-cultures (double positive events are represented in the upper right quadrant of each dot plot). Representative dot plots of NKp46+IFN-γ+ cells incubated with 5mg and 10mg lyophilized bacterial culture medium supernatant (CS) from Mb04-303 are depicted in line C.

From: Blanco FC, Gravisaco MJ, Bigi MM, García EA, Marquez C, McNeil M, Jackson M, Bigi F.
Identifying Bacterial and Host Factors Involved in the Interaction of Mycobacterium bovis with the Bovine Innate Immune Cells.
Front Immunol. 2021 Jul 15;12:674643.
doi: 10.3389/fimmu.2021.674643.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Bovine WC1:FITC

Product Type
Monoclonal Antibody
Clone
CC101
Isotype
IgG2a
Specificity
WC1

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Product Code Applications Pack Size List Price Your Price Qty
MCA1655F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Bovine WC1 antibody, clone CC101, recognizes a subset of WC1+ T-cells expressing the WC1.1 isoform (MacHugh et al. 1993).

The bovine WC1 cell surface antigen is expressed by a population of gamma/delta T-cells that lack CD2, CD4 and CD8, but express CD3. WC1 expression appears to be heterogeneous and antibodies to this cluster show differing reaction patterns (Crocker et al. 1993).

Mouse anti bovine WC1, clone CC101, immunoprecipitates a 215 kDa molecule from bovine cells and also recognizes the swine homolog of WC1, which is a 180 kDa molecule. In pigs, the 180 kDa molecule is expressed by a gamma/delta TCR positive T-cell population that also lack CD2, CD4 and CD8 (Carr et al. 1994).

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Con A stimulated bovine lymphocytes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
>
List Price Your Price
loader
Description Mouse IgG2a Negative Control:FITC

Source Reference

  1. Carr, M.M. et al. (1994) Expression on porcine gamma/delta lymphocytes of a phylogenetically conserved surface antigen previously restricted in expression to ruminant gamma/delta T lymphocytes.
    Immunology 81: 36-40.

References for WC1 antibody

  1. Howard, C.J. & Naessens, J. (1993) Summary of workshop findings for cattle.
    Vet Immunol Immunopathol. 39: 25-48.
  2. MacHugh, N. et al. (1993) Clustering of monoclonal antibodies recognizing different members of the WC1 gene family.
    Vet Immunol Immunopathol. 39: 155-60.
  3. Crocker, G. et al. (1993) Analysis of the gamma/delta T cell restricted antigen WC1.
    Vet Immunol Immunopathol. 39: 137-44.
  4. Lund, B. et al. (1993) Expression of T19 (WC1) molecules by ovine lymphocytes.
    Vet Immunol Immunopathol. 39: 145-53.
  5. Schröder, A.C. & Hamann, J. (2005) The influence of technical factors on differential cell count in milk.
    J Dairy Res. 72: 153-8.
  6. Patarroyo, J.H. et al. (2009) Immune response of bovines stimulated by synthetic vaccine SBm7462 against Rhipicephalus (Boophilus) microplus.
    Vet Parasitol. 166: 333-9.
  7. Al-Mohammed Salem Kazem, T. et al. (2012) The Cellular Populations of Normal Camel (<i>Camelus dromedaries</i>) Milk
    Open Journal of Veterinary Medicine. 02 (04): 262-5.
  8. Sedlak, C. et al. (2014) IL-12 and IL-18 induce interferon-γ production and de novo CD2 expression in porcine γδ T cells.
    Dev Comp Immunol. 47: 115-22.
    9. View The Latest Product References
  9. Liu, X. et al. (2014) Crusted scabies is associated with increased IL-17 secretion by skin T cells.
    Parasite Immunol. 36: 594-604.
  10. Al-Ashqar, R.A. et al. (2015) The CD markers of camel (Camelus dromedarius.) milk cells during mastitis: the LPAM-1 expression is an indication of possible mucosal nature of the cellular trafficking.
    Res Vet Sci. 99: 77-81.
  11. Heiser, A. et al. (2015) Grazing dairy cows had decreased interferon-γ, tumor necrosis factor, and interleukin-17, and increased expression of interleukin-10 during the first week after calving.
    J Dairy Sci. 98: 937-46.
  12. Herry, V. et al. (2017) Local immunization impacts the response of dairy cows to Escherichia coli mastitis.
    Sci Rep. 7 (1): 3441.
  13. Hussen, J. et al. (2018) Expression Patterns of Cell Adhesion Molecules on CD4+ T Cells and WC1+ T Cells in the Peripheral Blood of Dromedary Camels.
    Pakistan Veterinary Journal. 38 (03): 231-236.
  14. Kato-Mori, Y. et al. (2021) Characterization of a variant CD4 molecule in Japanese Black cattle.
    Vet Immunol Immunopathol. 232: 110167.
  15. Blanco, F.C. et al. (2021) Identifying Bacterial and Host Factors Involved in the Interaction of Mycobacterium bovis with the Bovine Innate Immune Cells.
    Front Immunol. 12: 674643.

Further Reading

  1. Wijngaard, P. et al. (1992) Molecular characterization of the WC1 antigen expressed specifically on bovine CD4-CD8- gamma delta T lymphocytes.
    J Immunol. 149: 3273-7.
  2. Takamatsu, H.H. et al. (2006) Porcine gammadelta T cells: possible roles on the innate and adaptive immune responses following virus infection.
    Vet Immunol Immunopathol. 112: 49-61.
  3. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

RRID
AB_324521
UniProt
P30205
Entrez Gene
CD163L1
GO Terms
GO:0016020 membrane
GO:0005576 extracellular region
GO:0005044 scavenger receptor activity

MCA1655F

163861 1709

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