CD69 antibody | H1.2F3

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat	1/10

Flow Cytometry

Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/BUF041B).

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Hamster (Armenian) IgG Negative Control:FITC	MCA2356F	F	0.1 mg		Log in	- +	>Add to Cart Get Quote Get Quote Add to Quote
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Description	Hamster (Armenian) IgG Negative Control:FITC

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Mouse Seroblock FcR	BUF041A	F	0.1 mg		Log in	- +	Add to Cart Add to Quote Get Quote
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Mouse Seroblock FcR	BUF041B	F	0.5 mg		Log in	- +	Add to Cart Add to Quote Get Quote
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Source Reference

1. Yokoyama, W.M. et al. (1988) Characterization of a cell surface-expressed disulfide-linked dimer involved in murine T cell activation.
   J Immunol. 141 (2): 369-76.

References for CD69 antibody

1. Iwashiro, M. et al. (2001) Immunosuppression by CD4+ regulatory T cells induced by chronic retroviral infection.
   Proc Natl Acad Sci U S A. 98 (16): 9226-30.
2. Karrer, U. et al. (2003) Memory inflation: continuous accumulation of antiviral CD8+ T cells over time.
   J Immunol. 170 (4): 2022-9.
3. Marshall-Clarke, S. et al. (2003) A differential requirement for phosphoinositide 3-kinase reveals two pathways for inducible upregulation of major histocompatibility complex class II molecules and CD86 expression by murine B lymphocytes.
   Immunology. 109 (1): 102-8.
4. Chalifour, A. et al. (2004) Direct bacterial protein PAMP recognition by human NK cells involves TLRs and triggers alpha-defensin production.
   Blood. 104 (6): 1778-83.
5. Cohen-sfady, M. et al. (2005) Heat shock protein 60 activates B cells via the TLR4-MyD88 pathway.
   J Immunol. 175 (6): 3594-602.
6. Hickman, H.D. et al. (2008) Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes.
   Nat Immunol. 9 (2): 155-65.
7. Kvakan, H. et al. (2009) Regulatory T cells ameliorate angiotensin II-induced cardiac damage.
   Circulation. 119 (22): 2904-12.

Flow Cytometry

Synonyms

AIM

RRID

AB_2075078

UniProt

P37217

Entrez Gene

Cd69

GO Terms

GO:0016021 integral to membrane

GO:0005529 sugar binding

GO:0009897 external side of plasma membrane

MCA1580FT

020614 162960 165233

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