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Lambda Light Chain antibody | Mc24-IC6

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Mouse anti Human Lambda Light Chain:RPE

Product Type
Monoclonal Antibody
Clone
Mc24-IC6
Isotype
IgG1
Specificity
Lambda Light Chain

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Product Code Applications Pack Size List Price Your Price Qty
MCA1579PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F 100 Tests loader
List Price Your Price
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Mouse anti Human Lambda Light Chain antibody, clone Mc24-IC6 recognizes human lambda light chains, and shows no cross- reactivity to kappa light chains or to immunoglobulin heavy chains.

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Purified human IgG.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl washed whole blood

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

References for Lambda Light Chain antibody

  1. Reynolds, W.M. et al. (1992) A simple technique for the determination of kappa and lambda immunoglobulin light chain expression by B cells in whole blood.
    J Immunol Methods. 151 (1-2): 123-9.
  2. Suárez, E. et al. (2004) Human monoclonal antibodies produced in transgenic BABkappa,lambda mice recognising idiotypic immunoglobulins of human lymphoma cells.
    Mol Immunol. 41 (5): 519-26.

RRID
AB_322910
UniProt
P0CG04
Entrez Gene
IGLV2-14
GO Terms
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response

MCA1579PE

INN1705

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with LAMBDA LIGHT CHAIN specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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