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TNF Alpha antibody | MP6-XT3

Rat anti Mouse TNF Alpha

Product Type
Monoclonal Antibody
Clone
MP6-XT3
Isotype
IgG1
Specificity
TNF Alpha

Product Code Applications Pack Size List Price Your Price Qty
MCA1487
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F * WB 0.5 mg loader
List Price Your Price
loader

Rat anti Mouse TNF alpha antibody, clone MP6-XT3 recognizes mouse tumor necrosis factor alpha (TNF alpha), also known as TNFSF2. This 17 kDa cytokine is produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26 kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immunoregulatory role.

Rat anti Mouse TNF alpha antibody, clone MP6-XT3 is reported to neutralize the effects of mouse TNF alpha (Bauman et al. 2003).

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant murine TNF-alpha expressed in E. coli.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml
Flow Cytometry 1
Western Blotting
  1. 1 Membrane permeabilisation is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl

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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
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References for TNF Alpha antibody

  1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
    Immunol Rev. 127: 5-24.
  2. Huffnagle, G.B. et al. (1996) Afferent phase production of TNF-alpha is required for the development of protective T cell immunity to Cryptococcus neoformans.
    J Immunol. 157: 4529-36.
  3. Bauman, S.K. et al. (2003) Effects of tumor necrosis factor alpha on dendritic cell accumulation in lymph nodes draining the immunization site and the impact on the anticryptococcal cell-mediated immune response.
    Infect Immun. 71 (1): 68-74.
  4. Reljic, R. et al. (2004) Mouse IgA inhibits cell growth by stimulating tumor necrosis factor-alpha production and apoptosis of macrophage cell lines.
    Int Immunol. 16 (4): 607-14.

RRID
AB_322498
UniProt
P06804
Entrez Gene
Tnf
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0001891 phagocytic cup
GO:0002876 positive regulation of chronic inflammatory response to antigenic stimulus
GO:0002925 positive regulation of humoral immune response mediated by circulating immunoglobulin
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0030141 stored secretory granule
GO:0006006 glucose metabolic process
GO:0006927 transformed cell apoptosis
GO:0006959 humoral immune response
GO:0007254 JNK cascade
GO:0009887 organ morphogenesis
GO:0050830 defense response to Gram-positive bacterium
GO:0008625 induction of apoptosis via death domain receptors
GO:0009897 external side of plasma membrane
GO:0030198 extracellular matrix organization
GO:0030316 osteoclast differentiation
GO:0031622 positive regulation of fever generation
GO:0032729 positive regulation of interferon-gamma production
GO:0032755 positive regulation of interleukin-6 production
GO:0033209 tumor necrosis factor-mediated signaling pathway
GO:0044130 negative regulation of growth of symbiont in host
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB cascade
GO:0045121 membrane raft
GO:0045670 regulation of osteoclast differentiation
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0045994 positive regulation of translational initiation by iron
GO:0046325 negative regulation of glucose import
GO:0046330 positive regulation of JNK cascade
GO:0051023 regulation of immunoglobulin secretion
GO:0051798 positive regulation of hair follicle development
GO:0055037 recycling endosome
GO:0060664 epithelial cell proliferation involved in salivary gland morphogenesis
GO:0060693 regulation of branching involved in salivary gland morphogenesis

MCA1487

154713

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