Browsing Modes for Business Users

CD8 Alpha/Beta antibody | vpg9

Mouse anti Cat CD8 alpha/beta:RPE

Product Type
Monoclonal Antibody
Clone
vpg9
Isotype
IgG1
Specificity
CD8 Alpha/Beta

Product Code Applications Pack Size List Price Your Price Qty
MCA1347PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader

Mouse anti Cat CD8 alpha/beta antibody, clone vpg9 recognizes the feline homolog of human CD8. Clone vpg 9 specifically recognizes an epitope associated with the alpha/beta complex of the CD8 heterodimer (Shimojima et al. 1998).

Target Species
Cat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Antibody purified from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Feline thymocytes.
Fusion Partners
Spleen cells from immunized BALB/c were fused with cells of the NS0 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA1209PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

References for CD8 Alpha/Beta antibody

  1. Willett, B.J., and Callanan, J.J. (1995) The expression of leucocyte differentiation antigens in the feline immune system.
    P 3-15. In B.J. Willett and O.J. Jarrett (eds). Feline Immunology and Immunodeficiency. Oxford University Press, Oxford.
  2. Callanan, J. et al. (1993) Morphologic characterization of the lymph node changes in feline immunodeficiency virus infection as an animal model of AIDS.
    In P. Racz, N. L. Letvin, and J.C. Gluckman (eds)., Animal models of HIV and other retroviral infections. S. Karger, Basel, Switzerland. P. 115-136.
  3. Shimojima, M. et al. (1998) Characterization of anti-feline CD8 monoclonal antibodies.
    Vet Immunol Immunopathol. 61 (1): 17-23.
  4. Veir, J.K. et al. (2007) Effect of supplementation with Enterococcus faecium (SF68) on immune functions in cats.
    Vet Ther. 8: 229-38.
  5. Pistello, M. et al. (2010) Env-expressing autologous T lymphocytes induce neutralizing antibody and afford marked protection against feline immunodeficiency virus.
    J Virol. 84: 3845-56.
  6. Willett, B.J. et al. (2007) Probing the interaction between feline immunodeficiency virus and CD134 by using the novel monoclonal antibody 7D6 and the CD134 (Ox40) ligand.
    J Virol. 81: 9665-79.
  7. Willett, B.J. et al. (2013) Selective expansion of viral variants following experimental transmission of a reconstituted feline immunodeficiency virus quasispecies.
    PLoS One. 8 (1): e54871.
  8. Milner, R.J. et al. (2004) Suppurative rhinitis associated with Haemophilus species infection in a cat.
    J S Afr Vet Assoc. 75 (2): 103-7.
  9. View The Latest Product References
  10. Rydzewski, L. et al. (2016) Identification of a novel feline large granular lymphoma cell line (S87) as non-MHC-restricted cytotoxic T-cell line and assessment of its genetic instability.
    Vet Immunol Immunopathol. 177: 24-34.
  11. de Oliveira Medeiros, S. et al. (2016) Follow-up on long-term antiretroviral therapy for cats infected with feline immunodeficiency virus.
    J Feline Med Surg. 18 (4): 264-72.
  12. Guzera, M. et al. (2016) The use of flow cytometry for immunophenotyping lymphoproliferative disorders in cats: a retrospective study of 19 cases.
    Vet Comp Oncol. 14 Suppl 1: 40-51.
  13. O'Brien, T. et al. (2015) Moderate dietary supplementation with vitamin E enhances lymphocyte functionality in the adult cat.
    Res Vet Sci. 99: 63-9.
  14. Sparger, E.E. et al. (2018) Investigation of immune cell markers in feline oral squamous cell carcinoma.
    Vet Immunol Immunopathol. 202: 52-62.
  15. Maeta, N. et al. (2019) Lymphokine-activated killer cell transplantation after anti-cancer treatment in two aged cats.
    Open Vet J. 9 (2): 147-50.
  16. Mustaffa-Kamal, F. et al. (2019) Characterization of antiviral T cell responses during primary and secondary challenge of laboratory cats with feline infectious peritonitis virus (FIPV).
    BMC Vet Res. 15 (1): 165.
  17. Rütgen, B.C. et al. (2022) Composition of lymphocyte subpopulations in normal and mildly reactive peripheral lymph nodes in cats.
    J Feline Med Surg. 24 (2): 77-90.
  18. Cha, S. et al. (2023) Non-B, Non-T Acute Lymphoblastic Leukemia in a Cat
    Journal of Veterinary Clinics. 40 (4): 298-302.

Flow Cytometry

RRID
AB_2229071
UniProt
P41688
P79336
Entrez Gene
CD8A
CD8B
GO Terms
GO:0016021 integral to membrane

MCA1347PE

1605

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with CD8 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Cat Products
Please note