CD79a antibody | ZL7-4
Mouse anti Human CD79a:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- ZL7-4
- Isotype
- IgG1
- Specificity
- CD79a
Antibody Quality and Performance Guarantee
| Mouse anti Human CD79a antibody, clone ZL7-4 recognizes the human B-cell antigen receptor complex-associated protein alpha chain, also known as MB-1 membrane glycoprotein or CD79a. clone ZL7-4 reacts with CD79a positive cells by flow cytometry and with CD79a in an ELISA specific for a fusion protein of CD79a-Fc. Mouse anti Human CD79a antibody, clone ZL7-4 has been reported to be useful in distinguishing B-CLL from mantle cell lymphoma in flow cytometric assays (Bell et al. 1999). Mouse anti Human CD79a antibody, clone ZL7-4 has been reported to be suitable for Immunohistochemistry on frozen and pre-treated paraffin sections, but does exhibit epithelial staining. Mouse anti Human CD79a antibody, clone ZL7-4 has been reported to induce phosphorylation of syk kinase (Lanham et al. 2003). |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- IgM complex isolated from Daudi cells.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- MCA1298A647T: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- MCA1298A647: The Alexa Fluor dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood We recommend incubation times of at least 30 minutes with this antibody.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
References for CD79a antibody
-
Zhang. L. et al. (1995) The development of anti-CD79 monoclonal antibodies for treatment of B-cell neoplastic disease.
Therapeutic Immunology 2:191-202 -
Bell, P.B. et al. (1999) CD79a detected by ZL7.4 separates chronic lymphocytic leukemia from mantle cell lymphoma in the leukemic phase.
Cytometry. 38 (3): 102-5. -
Cragg, M.S. et al. (2002) The alternative transcript of CD79b is overexpressed in B-CLL and inhibits signaling for apoptosis.
Blood. 100: 3068-76. -
Lanham, S. et al. (2003) Differential signaling via surface IgM is associated with VH gene mutational status and CD38 expression in chronic lymphocytic leukemia.
Blood. 101 (3): 1087-93. -
Allsup, D.J. et al. (2005) B-cell receptor translocation to lipid rafts and associated signaling differ between prognostically important subgroups of chronic lymphocytic leukemia.
Cancer Res. 65: 7328-37. -
Rahemtullah, A. et al. (2008) CD20+ T-cell lymphoma: clinicopathologic analysis of 9 cases and a review of the literature.
Am J Surg Pathol. 32 (11): 1593-607. -
Vendel,A.C. et al (2009) B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNK
J Immunol. 182: 1509-17.
- Synonyms
- MB-1
- RRID
- AB_322590
- UniProt
- P11912
- Entrez Gene
- CD79A
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0005771 multivesicular body
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
- GO:0030183 B cell differentiation
- GO:0042100 B cell proliferation
- GO:0045121 membrane raft
- GO:0050853 B cell receptor signaling pathway
- View More GO Terms
MCA1298A647T
154733
1610
MCA1298A647
154732
1608
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