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CD33 antibody | WM53

Mouse anti Human CD33:RPE

Product Type
Monoclonal Antibody
Clone
WM53
Isotype
IgG1
Specificity
CD33

Product Code Applications Pack Size List Price Your Price Qty
MCA1271PE
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SDS Safety Datasheet SDS
F 100 Tests loader
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Mouse anti Human CD33 antibody, clone WM53 recognizes the human CD33 cell surface glycoprotein. This antigen, considered to be specific for the myeloid lineage, has also been reported to be present on cells of lymphoid origin.

Mouse anti Human CD33 antibody, clone WM53 immunoprecipitates a protein of ~75 kDa from myeloid cells, a smaller protein of approximately 67 kDa has been observed in immunoprecipitates from lymphoid targets.

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
Human AML cells
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
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List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

Source Reference

  1. Favaloro, E.J. et al. (1987) Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, gp67 (CD-33).
    Dis Markers. 5 (4): 215-25.

References for CD33 antibody

  1. Favaloro, E.J. et al. (1988) Further characterization of human myeloid antigens (gp160,95; gp150; gp67): investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD-33.
    Br J Haematol. 69 (2): 163-71.
  2. Favaloro, E.J. et al. (1993) Differential expression of surface antigens on activated endothelium.
    Immunol Cell Biol. 71:571-81.
  3. Pietschmann, P. et al. (2000) Surface markers and transendothelial migration of dendritic cells from elderly subjects.
    Exp Gerontol. 35: 213-24.
  4. Vamvakopoulos, J. et al. (2002) Genetic control of IL-1beta bioactivity through differential regulation of the IL-1 receptor antagonist.
    Eur J Immunol. 32 (10): 2988-96.
  5. Vamvakopoulos, J.E. & Green, C. (2003) HMG-CoA reductase inhibition aborts functional differentiation and triggers apoptosis in cultured primary human monocytes: a potential mechanism of statin-mediated vasculoprotection.
    BMC Cardiovasc Disord. 3: 6.
  6. Dahl C et al. (2004) Human mast cells express receptors for IL-3, IL-5 and GM-CSF; a partial map of receptors on human mast cells cultured in vitro.
    Allergy. 59 (10): 1087-96.
  7. Lajaunias, F. et al. (2005) Constitutive repressor activity of CD33 on human monocytes requires sialic acid recognition and phosphoinositide 3-kinase-mediated intracellular signaling.
    Eur J Immunol. 35: 243-51.
  8. Lin, C.W. et al. (2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.
    Blood. 106 (10): 3567-74.
  9. View The Latest Product References
  10. Hernández-Caselles, T. et al. (2006) A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing.
    J Leukoc Biol. 79: 46-58.
  11. Biedermann, B. et al. (2006) Analysis of the CD33-related siglec family reveals that Siglec-9 is an endocytic receptor expressed on subsets of acute myeloid leukemia cells and absent from normal hematopoietic progenitors.
    Leuk Res. 31: 211-20.
  12. Yasukawa, T. et al. (2012) Simple detection of surface antigens on living cells by applying distinct cell positioning with negative dielectrophoresis.
    Anal Chem. 84 (20): 8830-6.
  13. McCormack E et al. (2013) Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.
    Blood. 121 (7): e34-42.
  14. Hu, Z. et al. (2016) Self-assembled nanoparticles based on folic acid modified carboxymethyl chitosan conjugated with targeting antibody
    J Wuhan Univ of Technol-Mater. Sci. Ed. 31 (2): 446-53.
  15. Hernández-Caselles T et al. (2019) CD33 (Siglec-3) Inhibitory Function: Role in the NKG2D/DAP10 Activating Pathway.
    J Immunol Res. 2019: 6032141.

RRID
AB_321664
UniProt
P20138
Entrez Gene
CD33
GO Terms
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
GO:0007267 cell-cell signaling
GO:0008285 negative regulation of cell proliferation

MCA1271PE

INN1709

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