Browsing Modes for Business Users

CD39 antibody | A1

ISO 9001 Quality Assurance Manufacturing | Print
Anti Human CD39 Antibody, clone A1 thumbnail image 1 Anti Human CD39 Antibody, clone A1 thumbnail image 2 Anti Human CD39 Antibody, clone A1 thumbnail image 3
Anti Human CD39 Antibody, clone A1 gallery image 1
Figure A. PE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Mouse IgG1 isotype control (MCA928F). Figure B. PE conjugated Mouse anti Human CD19 (MCA1940PE) and FITC conjugated Mouse anti Human CD39 (MCA1268F). All experiments performed on human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD39 Antibody, clone A1 gallery image 2
Published customer image:
Mouse anti human CD39, clone A1(MCA1268GA) used for the evaluation of CD39 expression on cultured mononuclear cells by flow cytometry.
Image caption:
Phenotype of Foxp3hi and Foxp3int CD4+CD25hi T cells induced by malaria-infected RBCs. After 8 days of iRBC:PBMC co-culture, immuno magnetic selection was used to isolate CD4+CD25hi (black line) and CD25− cells (grey filled). CD25 and Foxp3 expression in the two sorted populations was analysed by flow cytometry (A). mRNA levels for Foxp3, TGFβ1 and IL-10 were normalized on 18SrRNA levels for each donor. Data from 8 donors (Mean+/−SEM) are shown, presented as relative mRNA levels normalized on mRNA levels in CD4+CD25− T cells for each donor (A). PBMC of 3 donors were phenotyped on day 0 prior to iRBC-PBMC co-culture set up, and after 6 days of iRBC:PBMC co-culture (2:1 ratio). Dot plots show the gating strategies for Foxp3 expressing cells on day 0 and day 6, respectively (B). Representative histograms depicting isotype control staining (grey filled) and specific staining for various surface markers on CD25−Foxp3− and CD25hi Foxp3-expressing CD4 T cells (black line) from each respective culture are shown (B).

From: Scholzen A, Mittag D, Rogerson SJ, Cooke BM, Plebanski M (2009)
Plasmodium falciparum–Mediated Induction of Human CD25hiFoxp3hi CD4 T Cells Is Independent of Direct TCR Stimulation and Requires IL-2, IL-10 and TGFβ.
PLoS Pathog 5(8): e1000543.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Human CD39 Antibody, clone A1 gallery image 3
Published customer image:
Mouse anti human CD39, clone A1 (MCA1268GA) used for the evaluation of CD39 expression on cultured mononuclear cells by flow cytometry.
Image caption:
Phenotype and functional analysis of Foxp3hi and Foxp3int T cells in direct co-cultures or separated through a transwell. T cells from direct or transwell-separated iRBC co-cultures were harvested on day 6 of culture and either re-stimulated with PMA/ionomycin for intracellular cytokine staining, or phenotyped directly. Foxp3hi:Foxp3int ratios and the percentage of IFNγ or IL-4 producing cells within the CD25hiFoxp3int population were determined for 5 donors. Values for transwell-separated T cells (black bars) are expressed as fold change following normalization onto values in direct co-cultures (white bars) for each donor (Mean+/−SEM) and compared by paired Student's t-test (A). Freshly isolated CD25−Foxp3− and CD25+Foxp3+ CD4+ T cells (day 0) and CD4+CD25−Foxp3−, Foxp3int and Foxp3hi CD4+CD25hi T cells from direct or transwell separated cultures (day 6) from 3 donors were phenotyped for a wide range of markers, and expression levels for each surface marker within each population were normalized on isotype control staining, when analysed as median fluorescence intensity (MFI). Expression levels of surface markers on CD4+CD25−Foxp3− cells were comparable between conditions for each donor (data not shown). Thus, to compare the phenotype of day 0 CD25+Foxp3+ nTregs, and day 6 induced CD25hiFoxp3int and CD25hiFoxp3hi cells (black bars), expression levels are expressed as fold change (Mean+/−SEM) compared to CD4+CD25−Foxp3− cells (white bars, relative level = 1) for each donor and condition (B). Experiments were set up with a 2:1 iRBC:PBMC ratio.

From: Scholzen A, Mittag D, Rogerson SJ, Cooke BM, Plebanski M (2009)
Plasmodium falciparum–Mediated Induction of Human CD25hiFoxp3hi CD4 T Cells Is Independent of Direct TCR Stimulation and Requires IL-2, IL-10 and TGFβ.
PLoS Pathog 5(8): e1000543.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD39:RPE

Product Type
Monoclonal Antibody
Clone
A1
Isotype
IgG1
Specificity
CD39

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
MCA1268PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader

Mouse anti Human CD39, clone A1 recognizes the human CD39 cell surface antigen, a ~70-100 kDa molecule expressed on peripheral blood B cells, T cells and monocytes, and weakly expressed by granulocytes.

CD39 has intrinsic ecto-ATPase activity (Wang et al. 1996), and expression can be induced on T cells and increased on B cells, as a late activation antigen (Maliszewski et al. 1994).

Mouse anti Human CD39, clone A1 has been shown to block MHC independent target cell recognition by hapten-specific CTL (Stockl et al. 2001).

Target Species
Human
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
PHA activated human lymphocytes
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
This product should be stored undiluted.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
List Price Your Price
Description Human Seroblock
Human Seroblock BUF070B F 200 Test
List Price Your Price
Description Human Seroblock

Source Reference

  1. Aversa, G.G. et al. (1988) Detection of a late lymphocyte activation marker by A1, a new monoclonal antibody.
    Transplant Proc. 20 (1): 49-52.

References for CD39 antibody

  1. Waugh, J.A. et al. (1989) Staining of normal and rejecting kidney using the activation panel.
    In: Leucocyte Typing IV. White cell differentiation antigens. Edited by Knapp, W. et al.Oxford University Press. p485.
  2. Aversa, G.G. and Hall, B.M. (1989) Activation panel antigen expression on PBL activated by PHA or in MLR.
    In: Leucocyte Typing IV. White cell differentiation antigens. Edited by Knapp, W. et al. Oxford University Press, p.498.
  3. Aversa, G.G. et al. (1989) Use of monoclonal antibodies to study in vivo and in vitro-activated lymphocytes.
    Transplant Proc. 21 (1 Pt 1): 349-50.
  4. Stein, H. et al. (1989) Activated Section report.
    In: Leucocyte Typing IV. White cell differentiation antigens. Edited by Knapp, W. et al. Oxford University Press, p.387.
  5. Suranyi, M.G. et al. (1991) Lymphocyte adhesion molecules in T cell-mediated lysis of human kidney cells.
    Kidney Int. 39 (2): 312-9.
  6. Stöckl, J. et al. (2001) Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL.
    J Immunol. 167 (5): 2724-33.
  7. Borsellino, G. et al. (2007) Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression
    Blood. 110:1225-32.
  8. Glenn, J.R. et al. (2008) Raised levels of CD39 in leucocytosis result in marked inhibition of ADP-induced platelet aggregation via rapid ADP hydrolysis.
    Platelets. 19: 59-69.
    9. View The Latest Product References
  9. Loeuillet, C. et al. (2008) In vitro whole-genome analysis identifies a susceptibility locus for HIV-1.
    PLoS Biol. 6: e32.
  10. Alam, M.S. et al. (2009) CD73 is expressed by human regulatory T helper cells and suppresses proinflammatory cytokine production and Helicobacter felis-induced gastritis in mice.
    J Infect Dis. 199: 494-504.
  11. Scholzen, A. et al. (2009) Plasmodium falciparum-mediated induction of human CD25Foxp3 CD4 T cells is independent of direct TCR stimulation and requires IL-2, IL-10 and TGFbeta.
    PLoS Pathog. 5: e1000543.
  12. Mittag, D. et al. (2010) The effector T cell response to ryegrass pollen is counterregulated by simultaneous induction of regulatory T cells.
    J Immunol. 184: 4708-16.
  13. Rawstron, A.C. et al. (2010) Chronic lymphocytic leukaemia (CLL) and CLL-type monoclonal B-cell lymphocytosis (MBL) show differential expression of molecules involved in lymphoid tissue homing.
    Cytometry B Clin Cytom. 78 Suppl 1: S42-6.
  14. Moreno-Fernandez, M.E. et al. (2011) Regulatory T cells control HIV replication in activated T cells through a cAMP-dependent mechanism.
    Blood. 117: 5372-80.
  15. Häusler SF et al. (2014) Anti-CD39 and anti-CD73 antibodies A1 and 7G2 improve targeted therapy in ovarian cancer by blocking adenosine-dependent immune evasion.
    Am J Transl Res. 6 (2): 129-39.

Flow Cytometry

RRID
AB_324696
UniProt
P49961
Entrez Gene
ENTPD1
GO Terms
GO:0007596 blood coagulation
GO:0007155 cell adhesion
GO:0005887 integral to plasma membrane
GO:0005524 ATP binding
GO:0016787 hydrolase activity

MCA1268PE

INN1704

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with CD39 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Human Products
Please note