CD123 antibody | 6H6
Mouse anti Human CD123 antibody, clone 6H6 (MCA1263) used to assess CD123 expression on human bone marrow mast cells by flow cytometry.
Image caption:
Consistent surface expression of FcεRIα, CD123 and integrins on HBMMC cultured initially either with or without IL-3. HBMMC (either IL-3(+) or IL-3(−)) were labeled with specific mAb against FcεRIα, CD123, CD117 and integrins such as CD18, CD51, and CD54, or subtype-matched irrevalent mouse IgG (shaded area) subsequently labeled with FITC-conjugated rabbit anti-mouse immunoglobulins. Fluorescence intensity was analyzed by flow cytometry. Histograms with specific mAb were overlaid with those using the isotype-matched mouse IgG. Data are representative of three independent experiments that showed similar results.
From: Shimizu Y, Matsumoto K, Okayama Y, Sakai K, Maeno T, Suga T, Miura T, Takai S, Kurabayashi M, Saito H.
Interleukin-3 does not affect the differentiation of mast cells derived from human bone marrow progenitors.
Immunol Invest. 2008;37(1):1-17.
doi: 10.1080/08820130701741742..
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD123:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 6H6
- Isotype
- IgG1
- Specificity
- CD123
Antibody Quality and Performance Guarantee
| Mouse anti Human CD123 antibody, clone 6H6 recognizes the human interleukin-3 (IL-3) receptor alpha chain, also known as CD123. Mouse anti Human CD123 antibody, clone 6H6 is useful for monitoring expression of the IL-3 receptor on activated endothelial cells, monocytes and eosinophils, sorting bone marrow progenitor cells and purification of basophils. Mouse anti Human CD123 antibody, clone 6H6 does not neutralize the activity of the IL-3 receptor alpha chain. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
0.2% bovine serum albumin - Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 1 x 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Mouse IgG1 Negative Control:RPE | ||||||
References for CD123 antibody
-
Shimizu, Y. et al. (2008) Interleukin-3 does not affect the differentiation of mast cells derived from human bone marrow progenitors.
Immunol Invest.37: 1-17 -
Massone, C. et al. (2011) Immunophenotype of skin lymphocytic infiltrate in patients co-infected with Mycobacterium leprae and human immunodeficiency virus: a scenario dependent on CD8+ and/or CD20+ cells.
Br J Dermatol. 165: 321-8. -
Pistulli, R. et al. (2020) Characterization of dendritic cells in human and experimental myocarditis.
ESC Heart Fail. 7 (5): 2305-17.
Further Reading
-
Korpelainen, E.I. et al. (1993) The receptor for interleukin 3 is selectively induced in human endothelial cells by tumor necrosis factor alpha and potentiates interleukin 8 secretion and neutrophil transmigration.
Proc Natl Acad Sci U S A. 90 (23): 11137-41.
- Synonyms
- Interleukin 3 Receptor Alpha
- UniProt
- P26951
- Entrez Gene
- IL3RA
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004912 interleukin-3 receptor activity
MCA1263PE
158159
158562
167092
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