CD49d antibody | PS/2

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Flow Cytometry

Use 10μl of the suggested working dilution to label 10⁶ cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

Description	Product Code	Applications	Pack Size	List Price	Your Price	Quantity
Mouse Seroblock FcR	BUF041A	F	0.1 mg		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	Mouse Seroblock FcR
Mouse Seroblock FcR	BUF041B	F	0.5 mg		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	Mouse Seroblock FcR

References for CD49d antibody

1. Miyake, K. et al. (1991) Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis.
   J Exp Med. 173 (3): 599-607.
2. Miyake, K. et al. (1991) A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture.
   J Cell Biol. 114 (3): 557-65.
3. Andrew, D.P. et al. (1994) Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation.
   J Immunol. 153 (9): 3847-61.
4. Tchilian, E.Z. et al. (1997) Anti-alpha 4 integrin antibody induces apoptosis in murine thymocytes and staphylococcal enterotoxin B-activated lymph node T cells.
   Immunology. 92: 321-7.
5. Enghofer, M. et al. (1998) Lymphocyte transfer in streptozotocin-induced diabetes: adhesion of donor cells to islet endothelium.
   Am J Physiol. 274: E928-35.
6. Liu, Z.J. et al. (1999) A novel role for H-Ras in the regulation of very late antigen-4 integrin and VCAM-1 via c-Myc-dependent and -independent mechanisms.
   J Immunol. 163: 4901-8.
7. Tanneau, G.M. et al. (1999) Differential recruitment of T- and IgA B-lymphocytes in the developing mammary gland in relation to homing receptors and vascular addressins.
   J Histochem Cytochem. 47: 1581-92.
8. Fukuoka, M. et al. (2000) Antiadhesive function of 130-kd glycoform of CD43 expressed in CD4 T-lymphocyte clones and transfectant cell lines.
   Blood. 96: 4267-75.
View The Latest Product References
9. Hokibara, S. et al. (2000) Effects of monoclonal antibodies to adhesion molecules on eosinophilic myocarditis in Toxocara canis-infected CBA/J mice.
   Clin Exp Immunol. 114: 236-44.
10. Bowden, R.A. et al. (2002) Role of alpha4 integrin and VCAM-1 in CD18-independent neutrophil migration across mouse cardiac endothelium.
    Circ Res. 90: 562-9.
11. Hirata, T. et al. (2002) P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin.
    J Immunol. 169: 4307-13.
12. Maus, U.A. et al. (2004) Pneumolysin-induced lung injury is independent of leukocyte trafficking into the alveolar space.
    J Immunol. 173: 1307-12.
13. Ferrer, P. et al. (2005) Association between pterostilbene and quercetin inhibits metastatic activity of B16 melanoma.
    Neoplasia. 7: 37-47.
14. Eshghi, S. et al. (2007) Alpha4beta1 integrin and erythropoietin mediate temporally distinct steps in erythropoiesis: integrins in red cell development.
    J Cell Biol. 177: 871-80.
15. Vaz, R. et al. (2012) Fibronectin promotes migration, alignment and fusion in an in vitro myoblast cell model.
    Cell Tissue Res. 348: 569-78.
16. Zhang, Y. et al. (2012) Autotaxin through lysophosphatidic acid stimulates polarization, motility, and transendothelial migration of naive T cells.
    J Immunol. 189: 3914-24.
17. Gillberg, L. et al. (2013) Effective treatment of mouse experimental colitis by alpha 2 integrin antibody: comparison with alpha 4 antibody and conventional therapy.
    Acta Physiol (Oxf). 207: 326-36.
18. Omenetti, S. et al. (2015) Dysregulated intrahepatic CD4⁺ T-cell activation drives liver inflammation in ileitis-prone SAMP1/YitFc mice.
    Cell Mol Gastroenterol Hepatol. 1 (4): 406-19.
19. Chung, K.J. et al. (2017) A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity.
    Nat Immunol. 18 (6): 654-64.
20. Andrews, S.L. et al. (2023) SVEP1 influences monocyte to macrophage differentiation via integrin α4β1/α9β1 and Rho/Rac signalling.
    Biochim Biophys Acta Mol Cell Res. 1870 (6): 119479.

Flow Cytometry

Functional Assays

Immunohistology - Frozen

Synonyms

Integrin Alpha 4 Chain

VLA-4

RRID

AB_566805

UniProt

Q00651

Entrez Gene

Itga4

GO Terms

GO:0007157 heterophilic cell-cell adhesion

GO:0001968 fibronectin binding

GO:0001974 blood vessel remodeling

GO:0004872 receptor activity

GO:0008305 integrin complex

GO:0007159 leukocyte cell-cell adhesion

GO:0007229 integrin-mediated signaling pathway

GO:0007507 heart development

GO:0009897 external side of plasma membrane

View More GO Terms

GO:0016477 cell migration

GO:0060324 face development

GO:0060710 chorio-allantoic fusion

MCA1230A488

149999

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