CD42a antibody | GRP-P
Mouse anti Human CD42a
- Product Type
- Monoclonal Antibody
- Clone
- GRP-P
- Isotype
- IgG1
- Specificity
- CD42a
Antibody Quality and Performance Guarantee
| Mouse anti Human CD42a antibody, clone GRP-P recognizes the platelet GPIX glycoprotein, a 23kDa surface marker expressed by platelets and megakaryocytes. Platelet GPIX is also known as CD42a. The CD42 complex is the major platelet receptor for von Willebrand factor. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mink - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Human red blood cells and platelets.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/100 |
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD42a antibody
-
Eisbacher, M. et al. (2001) Inducible expression of the megakaryocyte-specific gene glycoprotein IX is mediated through an Ets binding site and involves upstream activation of extracellular signal-regulated kinase.
Cell Growth Differ. 12: 435-45. -
Sarma, J. et al. (2002) Increased platelet binding to circulating monocytes in acute coronary syndromes.
Circulation. 105 (18): 2166-71. -
Fox, S.C. et al. (2004) Quantitation of platelet aggregation and microaggregate formation in whole blood by flow cytometry.
Platelets. 15: 85-93. -
Harding, S.A. et al. (2004) Increased CD40 ligand and platelet-monocyte aggregates in patients with type 1 diabetes mellitus.
Atherosclerosis. 176: 321-5. -
Schuberth, H.J. et al. (2007) Reactivity of cross-reacting monoclonal antibodies with canine leukocytes, platelets and erythrocytes.
Vet Immunol Immunopathol. 119: 47-55. -
Harding, S.A. et al. (2007) Clopidogrel reduces platelet-leucocyte aggregation, monocyte activation and RANTES secretion in type 2 diabetes mellitus.
Heart. 92: 1335-7. -
Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
Vet Immunol Immunopathol. 119: 27-37. -
Din, J.N. et al. (2008) Dietary intervention with oil rich fish reduces platelet-monocyte aggregation in man.
Atherosclerosis. 197: 290-6.
View The Latest Product References
-
Vettore, S. et al. (2008) Novel point mutation in a leucine-rich repeat of the GPIbalpha chain of the platelet von Willebrand factor receptor, GPIb/IX/V, resulting in an inherited dominant form of Bernard-Soulier syndrome affecting two unrelated families: the N41H variant.
Haematologica. 93: 1743-7. -
Bournazos, S. et al. (2008) Monocyte functional responsiveness after PSGL-1-mediated platelet adhesion is dependent on platelet activation status.
Arterioscler Thromb Vasc Biol. 28: 1491-8. -
Din, J.N. et al. (2010) Effect of moderate walnut consumption on lipid profile, arterial stiffness and platelet activation in humans.
Eur J Clin Nutr. 2011 Feb;65(2):234-9. -
Perdomo, J. et al. (2011) Quinine-induced thrombocytopenia: drug-dependent GPIb/IX antibodies inhibit megakaryocyte and proplatelet production in vitro.
Blood. 117: 5975-86. -
Jamaly, S. et al. (2018) Elevated plasma levels of P-selectin glycoprotein ligand-1-positive microvesicles in patients with unprovoked venous thromboembolism.
J Thromb Haemost. May 31 [Epub ahead of print]. -
Tunströmer, K. et al. (2018) Quantification of Platelet Contractile Movements during Thrombus Formation.
Thromb Haemost. 118 (9): 1600-11.
- Synonyms
- GPIX
- RRID
- AB_321696
- UniProt
- P14770
- Entrez Gene
- GP9
- GO Terms
- GO:0005515 protein binding
- GO:0007155 cell adhesion
- GO:0005887 integral to plasma membrane
- GO:0007597 blood coagulation, intrinsic pathway
- GO:0030168 platelet activation
MCA1227
150048
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