CD42a antibody | GRP-P
Mouse anti Human CD42a:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- GRP-P
- Isotype
- IgG1
- Specificity
- CD42a
Antibody Quality and Performance Guarantee
| Mouse anti Human CD42a antibody, clone GRP-P recognizes the platelet GPIX glycoprotein, a 23kDa surface marker expressed by platelets and megakaryocytes. Platelet GPIX is also known as CD42a. The CD42 complex is the major platelet receptor for von Willebrand factor. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mink - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Human red blood cells and platelets.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- MCA1227A647: The Alexa Fluor dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
- MCA1227A647T: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/5 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human Seroblock | ||||||
References for CD42a antibody
-
Eisbacher, M. et al. (2001) Inducible expression of the megakaryocyte-specific gene glycoprotein IX is mediated through an Ets binding site and involves upstream activation of extracellular signal-regulated kinase.
Cell Growth Differ. 12: 435-45. -
Sarma, J. et al. (2002) Increased platelet binding to circulating monocytes in acute coronary syndromes.
Circulation. 105 (18): 2166-71. -
Fox, S.C. et al. (2004) Quantitation of platelet aggregation and microaggregate formation in whole blood by flow cytometry.
Platelets. 15: 85-93. -
Harding, S.A. et al. (2004) Increased CD40 ligand and platelet-monocyte aggregates in patients with type 1 diabetes mellitus.
Atherosclerosis. 176: 321-5. -
Schuberth, H.J. et al. (2007) Reactivity of cross-reacting monoclonal antibodies with canine leukocytes, platelets and erythrocytes.
Vet Immunol Immunopathol. 119: 47-55. -
Harding, S.A. et al. (2007) Clopidogrel reduces platelet-leucocyte aggregation, monocyte activation and RANTES secretion in type 2 diabetes mellitus.
Heart. 92: 1335-7. -
Aasted, B. et al. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
Vet Immunol Immunopathol. 119: 27-37. -
Din, J.N. et al. (2008) Dietary intervention with oil rich fish reduces platelet-monocyte aggregation in man.
Atherosclerosis. 197: 290-6.
View The Latest Product References
-
Vettore, S. et al. (2008) Novel point mutation in a leucine-rich repeat of the GPIbalpha chain of the platelet von Willebrand factor receptor, GPIb/IX/V, resulting in an inherited dominant form of Bernard-Soulier syndrome affecting two unrelated families: the N41H variant.
Haematologica. 93: 1743-7. -
Bournazos, S. et al. (2008) Monocyte functional responsiveness after PSGL-1-mediated platelet adhesion is dependent on platelet activation status.
Arterioscler Thromb Vasc Biol. 28: 1491-8. -
Din, J.N. et al. (2010) Effect of moderate walnut consumption on lipid profile, arterial stiffness and platelet activation in humans.
Eur J Clin Nutr. 2011 Feb;65(2):234-9. -
Perdomo, J. et al. (2011) Quinine-induced thrombocytopenia: drug-dependent GPIb/IX antibodies inhibit megakaryocyte and proplatelet production in vitro.
Blood. 117: 5975-86. -
Jamaly, S. et al. (2018) Elevated plasma levels of P-selectin glycoprotein ligand-1-positive microvesicles in patients with unprovoked venous thromboembolism.
J Thromb Haemost. May 31 [Epub ahead of print]. -
Tunströmer, K. et al. (2018) Quantification of Platelet Contractile Movements during Thrombus Formation.
Thromb Haemost. 118 (9): 1600-11.
- Synonyms
- GPIX
- RRID
- AB_324853
- UniProt
- P14770
- Entrez Gene
- GP9
- GO Terms
- GO:0005515 protein binding
- GO:0007155 cell adhesion
- GO:0005887 integral to plasma membrane
- GO:0007597 blood coagulation, intrinsic pathway
- GO:0030168 platelet activation
MCA1227A647
155045
165616
1704
MCA1227A647T
153812
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