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CD2 antibody | LT2

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Anti Human CD2 Antibody, clone LT2 thumbnail image 1 Anti Human CD2 Antibody, clone LT2 thumbnail image 2 Anti Human CD2 Antibody, clone LT2 thumbnail image 3 Anti Human CD2 Antibody, clone LT2 thumbnail image 4 Anti Human CD2 Antibody, clone LT2 thumbnail image 5 Anti Human CD2 Antibody, clone LT2 thumbnail image 6 Anti Human CD2 Antibody, clone LT2 thumbnail image 7 Anti Human CD2 Antibody, clone LT2 thumbnail image 8 Anti Human CD2 Antibody, clone LT2 thumbnail image 9 Anti Human CD2 Antibody, clone LT2 thumbnail image 10 Anti Human CD2 Antibody, clone LT2 thumbnail image 11 Anti Human CD2 Antibody, clone LT2 thumbnail image 12 Anti Human CD2 Antibody, clone LT2 thumbnail image 13 Anti Human CD2 Antibody, clone LT2 thumbnail image 14 Anti Human CD2 Antibody, clone LT2 thumbnail image 15 Anti Human CD2 Antibody, clone LT2 thumbnail image 16 Anti Human CD2 Antibody, clone LT2 thumbnail image 17 Anti Human CD2 Antibody, clone LT2 thumbnail image 18 Anti Human CD2 Antibody, clone LT2 thumbnail image 19 Anti Human CD2 Antibody, clone LT2 thumbnail image 20 Anti Human CD2 Antibody, clone LT2 thumbnail image 21 Anti Human CD2 Antibody, clone LT2 thumbnail image 22 Anti Human CD2 Antibody, clone LT2 thumbnail image 23 Anti Human CD2 Antibody, clone LT2 thumbnail image 24 Anti Human CD2 Antibody, clone LT2 thumbnail image 25 Anti Human CD2 Antibody, clone LT2 thumbnail image 26 Anti Human CD2 Antibody, clone LT2 thumbnail image 27 Anti Human CD2 Antibody, clone LT2 thumbnail image 28 Anti Human CD2 Antibody, clone LT2 thumbnail image 29 Anti Human CD2 Antibody, clone LT2 thumbnail image 30 Anti Human CD2 Antibody, clone LT2 thumbnail image 31
Anti Human CD2 Antibody, clone LT2 gallery image 1
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and SBV515 conjugated Mouse anti Human CD2 (MCA1194SBV515). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 2
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Blue 700 (STAR210SBB700). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 3
Figure A. PE-Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267P647). Figure B. PE-Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267P647). and StarBright Violet 670 conjugated Mouse anti Human CD2 (MCA1194SBV670). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 4
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD4 (MCA1267SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD4 (MCA1267SBB700) and StarBright Violet 440 conjugated Mouse anti Human CD2 (MCA1194SBV440). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 5
Figure A. PE conjugated Mouse anti Human CD4 (MCA1267PE). Figure B. PE conjugated Mouse anti Human CD4 (MCA1267PE) and StarBright Violet 610 conjugated Mouse anti Human CD2 (MCA1194SBV610). All experiments performed on red blood lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 6
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Violet 670 (STAR210SBV670). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 7
Figure A. PE Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267P647). Figure B. PE Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267P647) and StarBright Violet 570 conjugated Mouse anti Human CD2 (MCA1194SBV570). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 8
Figure A. Pacific Blue conjugated Mouse anti Human CD4 (MCA1267PB). Figure B. Pacific Blue conjugated Mouse anti Human CD4 (MCA1267PB) and StarBright Violet 710 conjugated Mouse anti Human CD2 (MCA1194SBV710). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 9
Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488). Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 (MCA1267A488) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Violet 790 (STAR210SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 10
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267A647) and StarBright Violet 790 conjugated Mouse anti Human CD38 (MCA1194SBV790). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 11
Figure A. PE conjugated Mouse anti Human CD4 (MCA1267PE). Figure B. PE conjugated Mouse anti Human CD4 (MCA1267PE) and StarBright Violet 475 conjugated Mouse anti Human CD2 (MCA1194SBV475). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 12
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267A647) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Violet 475 (STAR210SBV475). All experiments performed on red cell lysed human blood gated on live single cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 13
Figure A. PE conjugated Mouse anti Human CD4 (MCA1267PE). Figure B. PE conjugated Mouse anti Human CD4 (MCA1267PE) and StarBright UltraViolet 400 conjugated Mouse anti Human CD2 (MCA1194SBUV400). All experiments performed on red cell lysed human blood gated on live lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 14
Figure A. Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267A647). Figure B. Alexa Fluor 647 conjugated Mouse anti Human CD4 (MCA1267A647) and StarBright Violet 760 conjugated Mouse anti Human CD2 (MCA1194SBV760). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 15
Figure A. PE conjugated Mouse anti Human CD4 (MCA1267PE). Figure B. PE conjugated Mouse anti Human CD4 (MCA1267PE) and StarBright UltraViolet 665 conjugated Mouse anti Human CD2 (MCA1194SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 16
Figure A. StarBright Violet 710 conjugated Mouse anti Human CD4 (MCA1267SBV515). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD4 (MCA1267SBV515) and StarBright UltraViolet 665 conjugated Mouse anti Human CD2 (MCA1194SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 17
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515). Figure B StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright UltraViolet 665 (STAR210SBUV665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 18
Figure A. StarBright Blue 700 conjugated Mouse anti Human CD4 (MCA1267SBB700). Figure B. StarBright Blue 700 conjugated Mouse anti Human CD4 (MCA1267SBB700) and StarBright UltraViolet 445 conjugated Mouse anti Human CD2 (MCA1194SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 19
Figure A. Starbright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700). Figure B. Starbright Blue 700 conjugated Mouse anti Human CD3 (MCA463SBB700) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright UltraViolet 445 (STAR210SBUV445). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 20
Figure A. StarBright UltraViolet 400 conjugated Mouse anti Human CD4 (MCA1267SBUV400). Figure B. StarBright UltraViolet 400 conjugated Mouse anti Human CD4 (MCA1267SBUV400) and StarBright UltraViolet 795 conjugated Mouse anti Human CD2 (MCA1194SBUV795). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 21
Figure A. StarBright UltraViolet 795 conjugated Rat anti Mouse CD19 (MCA1439SBUV795). Figure B. StarBright UltraViolet 795 conjugated Rat anti Mouse CD19 (MCA1439SBUV795) and StarBright UltraViolet 510 conjugated Rat anti Mouse CD25 (MCA1260SBUV510). All experiments performed on mouse splenocytes treated with Concanavalin A for 24hrs gated on live single cells, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 22
Figure A. Starbright Violet 710 conjugated Mouse anti Human CD4 (MCA1267SBV710). Starbright Violet 710 conjugated Mouse anti Human CD4 (MCA1267SBV710) and StarBright UltraViolet 575 conjugated Mouse anti Human CD2 (MCA1194SBUV575). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 23
Figure A. StarBright Violet 790 conjugated Mouse anti Human CD4 (MCA1267SBV790). Figure B. StarBright Violet 790 conjugated Mouse anti Human CD4 (MCA1267SBV790) and StarBright UltraViolet 605 conjugated Mouse anti Human CD2 (MCA1194SBUV605). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 24
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515) and StarBright Blue 580 conjugated Mouse anti Human CD2 (MCA1194SBB580). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 25
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD4 (MCA1267SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD4 (MCA1267SBV440) and StarBright Blue 675 conjugated Mouse anti Human CD2 (MCA1194SBB675). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 26
Figure A. StarBright UltraViolet 665 conjugated Mouse anti Human CD4 (MCA1267SBUV665). Figure B. StarBright UltraViolet 665 conjugated Mouse anti Human CD4 (MCA1267SBUV665) and StarBright Blue 765 conjugated Mouse anti Human CD2 (MCA1194SBB765). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 27
Figure A. StarBright Violet 440 conjugated Mouse anti Human CD4 (MCA1267SBV440). Figure B. StarBright Violet 440 conjugated Mouse anti Human CD4 (MCA1267SBV440) and StarBright Blue 810 conjugated Mouse anti Human CD2 (MCA1194SBB810). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 28
Figure A. StarBright Violet 710 conjugated Mouse anti Human CD4 (MCA1267SBV670). Figure B. StarBright Violet 710 conjugated Mouse anti Human CD4 (MCA1267SBV670) and StarBright Yellow 575 conjugated Mouse anti Human CD2 (MCA1194SBY575). All experiments performed on red cell lysed Human blood gated on live single cell lymphocytes, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 29
Figure A. StarBright Blue 615 conjugated Mouse anti Human CD4 (MCA1267SBB615). Figure B. StarBright Blue 615 conjugated Mouse anti Human CD4 (MCA1267SBB615) and StarBright Yellow 720 conjugated Mouse anti Human CD2 (2). All experiments performed on human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 30
Figure A. StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515). Figure B. StarBright Violet 515 conjugated Mouse anti Human CD4 (MCA1267SBV515) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Yellow 605 (STAR210SBY605). All experiments performed on red cell lysed human blood gated on live single lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.
Anti Human CD2 Antibody, clone LT2 gallery image 31
Figure A. StarBright Yellow 665 conjugated Mouse anti Human CD3 (MCA463SBV570). Figure B. StarBright Yellow 665 conjugated Mouse anti Human CD3 (MCA463SBV570) and Biotin conjugated Mouse anti Human CD2 (MCA1194B) detected with Streptavidin StarBright Yellow 665 (STAR210SBY665). All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Human CD2:Low Endotoxin

Product Type
Monoclonal Antibody
Clone
LT2
Isotype
IgG2b
Specificity
CD2

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MCA1194EL
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C * F FN IP 0.5 mg loader
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Mouse anti Human CD2 antibody, clone LT2 recognizes human CD2, a single chain transmembrane glycoprotein of 50 kDa, expressed by T cells, NK cells and thymocytes.

CD2 is a receptor for CD58 (LFA-3), and is important in mediating the interaction between T cells and target cells or antigen presenting cells.

Mouse anti Human CD2 antibody, clone LT2 inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
Chimpanzee
Cynomolgus monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Normal human peripheral blood lymphocytes.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Endotoxin Level
<0.01 EU/ug
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20°C only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Functional Assays
Immunohistology - Frozen 1 1/200 1/400
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl
Histology Positive Control Tissue
Human tonsil

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Description Mouse IgG2b Negative Control

References for CD2 antibody

  1. Lin, C.W. et al. (2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.
    Blood. 106 (10): 3567-74.
  2. Kap, Y.S. et al. (2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
    J Histochem Cytochem. 57: 1159-67.
  3. Bruger, A.M. et al. (2020) Protocol to assess the suppression of T-cell proliferation by human MDSC.
    Methods Enzymol. 632: 155-92.

Further Reading

  1. Moingeon, P. et al. (1989) The structural biology of CD2.
    Immunol Rev. 111: 111-44.
  2. Beyers, A.D. et al. (1989) Activation of T lymphocytes via monoclonal antibodies against rat cell surface antigens with particular reference to CD2 antigen.
    Immunol Rev. 111: 59-77.

Synonyms
LFA-2
RRID
AB_2073672
UniProt
P06729
Entrez Gene
CD2
GO Terms
GO:0001766 membrane raft polarization
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0006917 induction of apoptosis
GO:0007166 cell surface receptor linked signaling pathway
GO:0016337 cell-cell adhesion
GO:0030101 natural killer cell activation
GO:0030887 positive regulation of myeloid dendritic cell activation
GO:0042110 T cell activation
GO:0045580 regulation of T cell differentiation
GO:0050900 leukocyte migration

MCA1194EL

163158 1706 1709

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