CD2 antibody | LT2
Mouse anti Human CD2:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- LT2
- Isotype
- IgG2b
- Specificity
- CD2
Mouse anti Human CD2 antibody, clone LT2 recognizes human CD2, a single chain transmembrane glycoprotein of 50 kDa, expressed by T cells, NK cells and thymocytes. CD2 is a receptor for CD58 (LFA-3), and is important in mediating the interaction between T cells and target cells or antigen presenting cells. Mouse anti Human CD2 antibody, clone LT2 inhibits NK cell mediated cytotoxicity, and ConA and PHA mediated blast transformation. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey Chimpanzee Cynomolgus monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Normal human peripheral blood lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63.653 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- MCA1194A647T: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- MCA1194A647: The Alexa Fluor dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays, and are covered by pending and issued patents.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl of whole blood using the lysed whole blood labeling method.
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG2b Negative Control:Alexa Fluor® 647 | MCA691A647 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG2b Negative Control:Alexa Fluor® 647 |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | Log in | |||
List Price | Your Price | ||||||
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Description | Human Seroblock | ||||||
Human Seroblock | BUF070B | F | 200 Test | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Human Seroblock |
References for CD2 antibody
-
Lin, C.W. et al. (2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.
Blood. 106 (10): 3567-74. -
Kap, Y.S. et al. (2009) A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates.
J Histochem Cytochem. 57: 1159-67. -
Bruger, A.M. et al. (2020) Protocol to assess the suppression of T-cell proliferation by human MDSC.
Methods Enzymol. 632: 155-92.
Further Reading
-
Moingeon, P. et al. (1989) The structural biology of CD2.
Immunol Rev. 111: 111-44. -
Beyers, A.D. et al. (1989) Activation of T lymphocytes via monoclonal antibodies against rat cell surface antigens with particular reference to CD2 antigen.
Immunol Rev. 111: 59-77.
- Synonyms
- LFA-2
- RRID
- AB_324865
- UniProt
- P06729
- Entrez Gene
- CD2
- GO Terms
- GO:0001766 membrane raft polarization
- GO:0007596 blood coagulation
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006917 induction of apoptosis
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0016337 cell-cell adhesion
- GO:0030101 natural killer cell activation
- GO:0030887 positive regulation of myeloid dendritic cell activation
- View More GO Terms
- GO:0042110 T cell activation
- GO:0045580 regulation of T cell differentiation
- GO:0050900 leukocyte migration
MCA1194A647T
153824MCA1194A647
155046 1706If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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