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CD71 antibody | DF1513

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Anti Human CD71 Antibody, clone DF1513 thumbnail image 1 Anti Human CD71 Antibody, clone DF1513 thumbnail image 2 Anti Human CD71 Antibody, clone DF1513 thumbnail image 3 Anti Human CD71 Antibody, clone DF1513 thumbnail image 4
Anti Human CD71 Antibody, clone DF1513 gallery image 1
Staining of MOLT4 ( human acute lymphoblastic leukemia) cells with Mouse anti Human CD71: FITC (MCA1148F)
Anti Human CD71 Antibody, clone DF1513 gallery image 2
Staining of Jurkat cells with Mouse anti Human CD71:RPE (MCA1148PE)
Anti Human CD71 Antibody, clone DF1513 gallery image 3
Staining of KG1 cells with Mouse anti Human CD71:Alexa Fluor® 647 (MCA1148A647)
Anti Human CD71 Antibody, clone DF1513 gallery image 4
Published customer image:
Mouse anti Human CD71 antibody, clone DF1513 (MCA1148) used to evaluate CD71 expression on erythroid cells during differentiation by flow cytometry.
Image caption:
Level of RBC membrane proteins in BEL-A compared to normal adult erythroid cells during erythropoiesis.
BEL-A cells in expansion medium (undif), and at day 4 and 10 following transfer to differentiation media, and erythroblasts differentiated from adult peripheral blood CD34+ cells at day 5, 12 and 21 in culture were incubated with antibodies to the proteins indicated and analysed by Flow Cytometry. Histograms illustrating fluorescent intensity of BEL-A cells incubated with antibodies to indicated proteins at labelled stages of differentiation. Percentages refer to percentage positivity.

From: Trakarnsanga K, Griffiths RE, Wilson MC, Blair A, Satchwell TJ, Meinders M, Cogan N, Kupzig S, Kurita R, Nakamura Y, Toye AM, Anstee DJ, Frayne J.
An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.
Nat Commun. 2017 Mar 14;8:14750.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD71:RPE

Product Type
Monoclonal Antibody
Clone
DF1513
Isotype
IgG1
Specificity
CD71

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Product Code Applications Pack Size List Price Your Price Qty
MCA1148PET
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 25 Tests loader
List Price Your Price
loader
MCA1148PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader

Mouse anti Human CD71 antibody, clone DF1513 recognizes the human CD71 cell surface antigen, a ~190 kDa homodimeric glycoprotein expressed by proliferating cells. CD71 is also known as the transferrin receptor. Mutation of the TFRC gene has been implicated in the development of Immunodeficiency 46 (IMD46) a combined immunodeficiency characterized by early onset chronic diarrhea, recurrent infections and intermittent neutropenia and thrombocytopenia (Jabara et al. 2016).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
Mustelid
Ferret
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
MCA1148PET: Reconstitute in 0.25 ml distilled water
MCA1148PE: Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
KGI cell line.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Pack Size: 25 Tests
Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Pack Size: 100 Tests
Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
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Description Mouse IgG1 Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Human Seroblock BUF070A F 50 Test
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Description Human Seroblock
Human Seroblock BUF070B F 200 Test
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Description Human Seroblock

References for CD71 antibody

  1. Sopper, S. et al. (1997) Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys.
    Cytometry. 29 (4): 351-62.
  2. Janes, P.W. et al. (1999) Aggregation of lipid rafts accompanies signaling via the T cell antigen receptor.
    J Cell Biol. 147: 447-61.
  3. Weissgerber, P. et al. (2003) Investigation of mechanisms involved in phagocytosis of Legionella pneumophila by human cells.
    FEMS Microbiol Lett. 219 (2): 173-9.
  4. Stockwin, L.H. et al. (2009) Artemisinin dimer anticancer activity correlates with heme-catalyzed reactive oxygen species generation and endoplasmic reticulum stress induction.
    Int J Cancer. 125: 1266-75.
  5. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
    Vet Immunol Immunopathol. 132:109-15.
  6. Meng, J. et al. (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
    PLoS One. 6(3):e17454.
  7. Procaccini, C. et al. (2012) Leptin-induced mTOR activation defines a specific molecular and transcriptional signature controlling CD4+ effector T cell responses.
    J Immunol. 189: 2941-53.
  8. Makoveichuk, E. et al. (2012) Inactivation of lipoprotein lipase occurs on the surface of THP-1 macrophages where oligomers of angiopoietin-like protein 4 are formed.
    Biochem Biophys Res Commun. 425:138-43.
    9. View The Latest Product References
  9. Trakarnsanga, K. et al. (2017) An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.
    Nat Commun. 8: 14750.
  10. Deng, K. et al. (2023) Application of In vitro transcytosis models to brain targeted biologics.
    PLoS One. 18 (8): e0289970.

Flow Cytometry

Synonyms
Transferrin Receptor
RRID
AB_1125285
UniProt
P02786
Entrez Gene
TFRC
GO Terms
GO:0008233 peptidase activity
GO:0004998 transferrin receptor activity
GO:0005887 integral to plasma membrane
GO:0005768 endosome
GO:0005905 coated pit
GO:0006508 proteolysis
GO:0006879 cellular iron ion homeostasis
GO:0006897 endocytosis
GO:0033572 transferrin transport
GO:0042470 melanosome
GO:0044419 interspecies interaction between organisms
GO:0055085 transmembrane transport

MCA1148PET

154033 INN1701

MCA1148PE

INN1701

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