CD66acd antibody | YTH71.3
Rat anti Human CD66acd
- Product Type
- Monoclonal Antibody
- Clone
- YTH71.3
- Isotype
- IgG2a
- Specificity
- CD66acd
Antibody Quality and Performance Guarantee
| Rat anti Human CD66acd antibody, clone YTH71.3 recognizes 3 members of the human CD66 family, namely CD66a, also known as Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein 1 or CEACAM1. Clone YTH71.3 also recognizes CD66c, known as Carcinoembryonic antigen-related cell adhesion molecule 6 or Non-specific crossreacting antigen. In addition the clone also recognizes CD66d which in turn is also known as Carcinoembryonic antigen-related cell adhesion molecule 3 or CGM1 (Watt et al. 1991). CD66 family members are single pass, type 1 membrane glycoproteins bearing a single V-type Ig-like domain and a varying number of C2-type Ig-like domains. Clone YTH71.3 recognizes epitopes in the N-terminal domain of these CD66 family members. CD66 members are expressed by human neutrophils and cells of the colon where it is down regulated in some cases of colon carcinoma (Neumaier et al. 2012). CD66a is also expressed in the human alveolar adenocarcinoma cell line A549 and may play a role in Moraxella catarrhalis induced apoptosis of pulmonary epithlial cells in diseases including COPD and emphysema (N'Guessan et al. 2007). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Human lymphocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
||
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
10 ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in his/her own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl.
References for CD66acd antibody
-
Jonker, M. et al. (1989) Reactivity of Mab specific for human CD markers with Rhesus monkey leucocytes.
Leucocyte Typing IV p 1058-1063 Oxford University Press, Oxford. -
Watt, S.M. et al. (1991) CD66 identifies a neutrophil-specific epitope within the haematopoietic system that is expressed by members of the carcinoembryonic family of adhesion molecules.
Blood 78: 63-74. -
Skubitz, K.M. et al. (1993) In Leucocyte Typing V. White Cell Differentiation Antigen p. 889 - 899 Volume One.
Oxford University Press, Oxford. -
Hill, D.J. and Virji, M. (2003) A novel cell-binding mechanism of Moraxella catarrhalis ubiquitous surface protein UspA: specific targeting of the N-domain of carcinoembryonic antigen-related cell adhesion molecules by UspA1.
Mol Microbiol. 48: 117-29. -
Bradley, C.J. et al. (2005) Critical determinants of the interactions of capsule-expressing Neisseria meningitidis with host cells: the role of receptor density in increased cellular targeting via the outer membrane Opa proteins
Cell Microbiol. 7: 1490-503. -
N'Guessan, P.D. et al. (2007) The UspA1 protein of Moraxella catarrhalis induces CEACAM-1-dependent apoptosis in alveolar epithelial cells.
J Infect Dis. 195: 1651-60.
- RRID
- AB_323396
- UniProt
- P13688
- P40198
- P40199
- Entrez Gene
- CEACAM1
- CEACAM3
- CEACAM6
- GO Terms
- GO:0016021 integral to membrane
- GO:0007165 signal transduction
- GO:0005887 integral to plasma membrane
- GO:0007267 cell-cell signaling
- GO:0031225 anchored to membrane
MCA1147G
148577
160976
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