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CD102 antibody | B-T1

Product Code Applications Pack Size List Price Your Price Qty
MCA1140
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF IP WB 0.2 mg loader
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loader

Mouse anti Human CD102 antibody, clone B-T1 recognizes human Intercellular adhesion molecule 2, also known as CD102 or ICAM-2. CD102 is a 275 amino acid ~55-65 kDa single pass type-1 transmembrane glycoprotein containing two Ig-like C2-type domains.

Mouse anti Human CD102 antibody, clone B-T1 inhibits cell adhesion (Xie et al. 1995)and T cell activation and also recognizes soluble ICAM-2.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Immunogen
ICAM-2 transfected CHO cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c (Iffa Credo) mice were fused with cells of the X63/Ag.8653 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20 1/50
Immunofluorescence
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

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References for CD102 antibody

  1. Xie, J. et al. (1995) Intercellular adhesion molecule-2 (CD102) binds to the leukocyte integrin CD11b/CD18 through the A domain.
    J Immunol. 155 (7): 3619-28.
  2. Jolly, C. et al. (2007) Adhesion molecule interactions facilitate human immunodeficiency virus type 1-induced virological synapse formation between T cells.
    J Virol. 81 (24): 13916-21.
  3. Huang, M.T. et al. (2005) Endothelial intercellular adhesion molecule (ICAM)-2 regulates angiogenesis.
    Blood. 106:1636-43.
  4. Birdsey, G.M. et al. (2008) Transcription factor Erg regulates angiogenesis and endothelial apoptosis through VE-cadherin.
    Blood. 111: 3498-506.
  5. Starke, R.D. et al. (2011) Endothelial von Willebrand factor regulates angiogenesis.
    Blood. 117: 1071-80.
  6. Ishido, S. et al. (2000) Inhibition of natural killer cell-mediated cytotoxicity by Kaposi's sarcoma-associated herpesvirus K5 protein.
    Immunity. 13: 365-74.
  7. Fricke. B. et al. (2005) Stomatin is mis-trafficked in the erythrocytes of overhydrated hereditary stomatocytosis, and is absent from normal primitive yolk sac-derived erythrocytes.
    Br J Haematol.131: 265-77.
  8. Garbe, Y. et al. (2011) Semiallogenic fusions of MSI(+) tumor cells and activated B cells induce MSI-specific T cell responses.
    BMC Cancer. 11: 410.
  9. View The Latest Product References
  10. McLaughlin, F. et al. (1998) Tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta down-regulate intercellular adhesion molecule (ICAM)-2 expression on the endothelium.
    Cell Adhes Commun. 6 (5): 381-400.

Immunofluorescence

Western Blotting

Synonyms
ICAM-2
RRID
AB_321980
UniProt
P13598
Entrez Gene
ICAM2
GO Terms
GO:0005887 integral to plasma membrane
GO:0005178 integrin binding
GO:0016337 cell-cell adhesion
GO:0050776 regulation of immune response

MCA1140

150746 164984

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