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IL-6 antibody

Rabbit anti Chicken Interleukin-6:Preservative Free

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IL-6

Product Code Applications Pack Size List Price Your Price Qty
AHP942Z
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E FN WB 0.5 mg loader
List Price Your Price
loader

Rabbit anti Chicken interleukin-6 antibody recognizes chicken Interleukin 6 (chIL-6).

Mammalian IL-6 is a multifunctional pro-inflammatory cytokine, which plays a major role in the regulation of immune responses, haematopoiesis and the induction of acute phase responses. The action of IL-6 occurs via the signal transducing receptor chain gp130 and the IL-6 receptor alpha chain is responsible for ligand binding.

IL-6 is produced by a wide variety of cells including B lymphocytes, T lymphocytes, monocytes/macrophages, fibroblasts and endothelial cells and has a widespread cellular effect, including B and T lymphocytes, hepatocytes and cells of the CNS.

Chicken hepatocyte-stimulating factor (HSF), secreted by lipopolysaccharide (LPS)-stimulated macrophages, induces fibronectin production in cultured chicken hepatocytes, and that HSF produced by LPS-treated fibroblasts, was neutralized by an antiserum to recombinant HuIL-6 (rHuIL-6). rHuIL-6 has also been shown as biologically active on chicken hepatocytes (Samad et al. 1993).

Rabbit anti Chicken interleukin-6 antibody ( AHP942Z) neutralizes chicken IL-6 in the murine 7TD1 IL-6 bioassay.

Target Species
Chicken
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to chicken Interleukin 6 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant chicken IL-6 (PAP003)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP942Z detects a band of approximately 22kDa.

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References for IL-6 antibody

  1. De Boever, S. et al. (2010) Flow cytometric differentiation of avian leukocytes and analysis of their intracellular cytokine expression.
    Avian Pathol. 39: 41-6.

Further Reading

  1. Staeheli, P. et al. (2001) Cytokines of birds: conserved functions--a largely different look.
    J Interferon Cytokine Res. 21 (12): 993-1010.
  2. Schneider, K. et al. (2001) Chicken interleukin-6. cDNA structure and biological properties.
    Eur J Biochem. 268 (15): 4200-6.
  3. Amrani, D.L. et al. (1986) Effect of hepatocyte-stimulating factor and glucocorticoids on plasma fibronectin levels.
    Biochem J. 238 (2): 365-71.
  4. Samad, F. et al. (1993) Stimulation of chick hepatocyte fibronectin production by fibroblast-conditioned medium is due to interleukin 6.
    Biochim Biophys Acta. 1181 (3): 207-13.

RRID
AB_2127753
UniProt
Q90YI0
Entrez Gene
IL6
GO Terms
GO:0006953 acute-phase response
GO:0005125 cytokine activity
GO:0005138 interleukin-6 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity

AHP942Z

153732

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