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CaMK II Alpha (pThr286) antibody

Rabbit anti CaMK II Alpha (pThr286)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CaMK II Alpha (pThr286)

Product Code Applications Pack Size List Price Your Price Qty
AHP2562
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 50 µl loader
List Price Your Price
loader

Rabbit anti CaMK II alpha (pThr286) antibody recognizes calcium/calmodulin-dependent protein kinase type II subunit alpha (CaMK II alpha) also known as CaM kinase II subunit alpha, when phosphorylated at threonine 286. CaMK II alpha is a member of the CAMK serine/threonine protein kinase family involved in the central nervous system, neurotransmitter release and synaptic plasticity.

CaMK II alpha is activated upon binding of calcium/calmodulin, resulting in autophosphorylation at threonine 286.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to CaMK II alpha (pThr286) was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.05% ProClin 300
50% Glycerol
Immunogen
Phospho specific-peptide corresponding to residues surrounding threonine 286 of human CaMK II alpha
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.

Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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UniProt
Q9UQM7
Entrez Gene
CAMK2A
GO Terms
GO:0005829 cytosol
GO:0004683 calmodulin-dependent protein kinase activity
GO:0005516 calmodulin binding
GO:0005524 ATP binding
GO:0005654 nucleoplasm
GO:0006468 protein phosphorylation
GO:0007268 synaptic transmission
GO:0030054 cell junction
GO:0030666 endocytic vesicle membrane
GO:0042734 presynaptic membrane
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0060333 interferon-gamma-mediated signaling pathway

AHP2562

141524

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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