DFF-45 antibody
Rabbit anti DFF-45
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- DFF-45
Rabbit anti DFF-45 antibody recognizes DFF, also known as DNA fragmentation factor subunit alpha, DFF1, DFFA, inhibitor of caspase-activated DNase or ICAD. DNA fragmentation factor (DFF) is a heterodimeric protein of 40kDa (DFF-40) and 45 kDa (DFF-45) subunits. During apoptosis caspase-3 cleaves DFF into the DFF-45 and DFF-40 fragments. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Expected from Sequence Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to DFF-45 was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Recombinant human DFF-45
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Paraffin | 1/50 | 1/200 | |
Western Blotting | 1/500 | 1/2000 |
- Western Blotting
- Rabbit anti DFF-45 antibody detects a band of approximately 43 kDa in cell lysates and tissue lysates under reducing conditions
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- UniProt
- O00273
- Entrez Gene
- DFFA
- GO Terms
- GO:0005886 plasma membrane
- GO:0005739 mitochondrion
- GO:0004536 deoxyribonuclease activity
- GO:0005829 cytosol
- GO:0035556 intracellular signal transduction
- GO:0005654 nucleoplasm
- GO:0006309 DNA fragmentation involved in apoptotic nuclear change
- GO:0042802 identical protein binding
- GO:0043066 negative regulation of apoptosis
- View More GO Terms
AHP2459
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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