ATG3 antibody | OTI3C6
KO Validated
IP-MS Validated
Mouse anti ATG3
- Product Type
- PrecisionAb Monoclonal
- Clone
- OTI3C6
- Isotype
- IgG2a
- Format
- Purified
- Specificity
- ATG3
Antibody Quality and Performance Guarantee
| Mouse anti Human ATG3 antibody recognizes the ubiquitin-like-conjugating enzyme ATG3, also known as 2610016C12Rik, ATG3 autophagy related 3 homolog, autophagy-related protein 3, hApg3, ubiquitin-like-conjugating enzyme ATG3. A product of the ATG3 gene, ubiquitin-like-conjugating enzyme ATG3 is a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of ATG3 is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms (provided by RefSeq, Jul 2013). Mouse anti Human ATG3 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. |
|
- Target Species
- Human
- Western Blotting
- Anti ATG3 detects a band of approximately 40 kDa in Jurkat cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human ATG3 (NP_071933) produced in HEK293T cells
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml.
- Regulatory
- For research purposes only.
- Guarantee
- 12 months from date of despatch.
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
Store undiluted at -20°C, avoiding repeated freeze thaw cycles.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoprecipitation |  |
||
| Western Blotting | |
1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG (H/L):HRP | ||||||
- UniProt
- Q9NT62
- Entrez Gene
- ATG3
- GO Terms
- GO:0000045 autophagic vacuole assembly
- GO:0000153 cytoplasmic ubiquitin ligase complex
- GO:0005829 cytosol
- GO:0016567 protein ubiquitination
- GO:0006612 protein targeting to membrane
- GO:0019899 enzyme binding
- GO:0019776 Atg8 ligase activity
- GO:0019777 Atg12 ligase activity
- GO:0043653 mitochondrial fragmentation involved in apoptosis
- View More GO Terms
VMA00280
151112
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