IL-1RA antibody
Rabbit anti Human Interleukin-1RA:Biotin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-1RA
Rabbit anti Human interleukin-1 receptor antagonist antibody recognises human Interleukin-1 receptor antagonist (IL-1RA), a naturally occurring inflammatory inhibitor protein, produced by monocytes, macrophages, neutrophils, corneal epithelial cells and fibroblasts, which inhibits the activity of IL-1 alpha and IL-1 beta, by competitively blocking binding to cell surface receptors for IL-1. Genetic variations in IL-1RA gene (IL1RN) are associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4), and IL-1RA has therapeutic applications in rheumatoid arthritis, asthma, psoriasis, sepsis, chronic myelogenous leukaemia and inflammatory bowel disease. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antiserum to human IL-1RA were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-1RA (PHP080)
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 0.25 | 1.0ug/ml | |
Western Blotting | 0.1 | 0.2ug/ml |
- ELISA
- This biotinylated human IL-1RA antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with a purified human IL-1RA antibody (AHP2184) as the capture reagent and recombinant human IL-1RA (PHP080) as the standard.
- Western Blotting
- This biotinylated human IL-1RA antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human IL-1RA (PHP080) as the positive control.
- Synonyms
- Interleukin-1 Receptor Antagonist
- RRID
- AB_10848349
- UniProt
- P18510
- Entrez Gene
- IL1RN
- GO Terms
- GO:0005886 plasma membrane
- GO:0005125 cytokine activity
- GO:0005152 interleukin-1 receptor antagonist activity
- GO:0005615 extracellular space
- GO:0005634 nucleus
- GO:0005813 centrosome
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0051384 response to glucocorticoid stimulus
- View More GO Terms
AHP2184B
152358 160804 166832If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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