TGF Beta antibody

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/2000	1/10000
Immunohistology - Paraffin 1			5ug/ml
Western Blotting		1/200	1/1000

1. ¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Histology Positive Control Tissue

Human spleen

Description	Product Code	Applications	Pack Size	List Price	Your Price	Quantity
Antigen Retrieval Buffer, pH8.0	BUF025A	P	500 ml		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB *	0.5 ml		Log in	- +	Add to Cart Add to Quote Get Quote
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Description	TidyBlot Western Blot Detection Reagent:HRP

References for TGF Beta antibody

1. Geng, H. et al. (2012) Lysophosphatidic acid increases proximal tubule cell secretion of profibrotic cytokines PDGF-B and CTGF through LPA2- and Gαq-mediated Rho and αvβ6 integrin-dependent activation of TGF-β.
   Am J Pathol. 181: 1236-49.
2. Polichnowski, A.J. et al. (2014) Severe renal mass reduction impairs recovery and promotes fibrosis after AKI.
   J Am Soc Nephrol. 25 (7): 1496-507.
3. Moore, L.B. et al. (2015) Loss of monocyte chemoattractant protein-1 alters macrophage polarization and reduces NFκB activation in the foreign body response.
   Acta Biomater. 11: 37-47.
4. Tark, K.C. et al. (2015) Effects of ginsenoside Rb1 on hypertrophic scar remodeling in rabbit model.
   Eur J Pharmacol. 750C: 151-59.

Immunohistology - Paraffin

Western Blotting

RRID

AB_2222443

UniProt

P01137

Entrez Gene

TGFB1

GO Terms

GO:0016049 cell growth

GO:0001933 negative regulation of protein phosphorylation

GO:0002062 chondrocyte differentiation

GO:0002244 hemopoietic progenitor cell differentiation

GO:0002248 connective tissue replacement involved in inflammatory response wound healing

GO:0002576 platelet degranulation

GO:0005114 type II transforming growth factor beta receptor binding

GO:0005578 proteinaceous extracellular matrix

GO:0005615 extracellular space

View More GO Terms

GO:0005634 nucleus

GO:0005796 Golgi lumen

GO:0006611 protein export from nucleus

GO:0006754 ATP biosynthetic process

GO:0006917 induction of apoptosis

GO:0007050 cell cycle arrest

GO:0007173 epidermal growth factor receptor signaling pathway

GO:0007179 transforming growth factor beta receptor signaling pathway

GO:0007183 SMAD protein complex assembly

GO:0007184 SMAD protein import into nucleus

GO:0007435 salivary gland morphogenesis

GO:0048535 lymph node development

GO:0008083 growth factor activity

GO:0008156 negative regulation of DNA replication

GO:0010718 positive regulation of epithelial to mesenchymal transition

GO:0010742 macrophage derived foam cell differentiation

GO:0010763 positive regulation of fibroblast migration

GO:0010800 positive regulation of peptidyl-threonine phosphorylation

GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation

GO:0016202 regulation of striated muscle tissue development

GO:0016563 transcription activator activity

GO:0017015 regulation of transforming growth factor beta receptor signaling pathway

GO:0019049 evasion of host defenses by virus

GO:0019058 viral infectious cycle

GO:0022408 negative regulation of cell-cell adhesion

GO:0030168 platelet activation

GO:0030308 negative regulation of cell growth

GO:0030501 positive regulation of bone mineralization

GO:0031093 platelet alpha granule lumen

GO:0031575 mitotic cell cycle G1/S transition checkpoint

GO:0032355 response to estradiol stimulus

GO:0032570 response to progesterone stimulus

GO:0032740 positive regulation of interleukin-17 production

GO:0032801 receptor catabolic process

GO:0032967 positive regulation of collagen biosynthetic process

GO:0033138 positive regulation of peptidyl-serine phosphorylation

GO:0035307 positive regulation of protein dephosphorylation

GO:0043406 positive regulation of MAP kinase activity

GO:0043536 positive regulation of blood vessel endothelial cell migration

GO:0043537 negative regulation of blood vessel endothelial cell migration

GO:0043552 positive regulation of phosphatidylinositol 3-kinase activity

GO:0043932 ossification involved in bone remodeling

GO:0045216 cell-cell junction organization

GO:0045599 negative regulation of fat cell differentiation

GO:0045930 negative regulation of mitotic cell cycle

GO:0046732 active induction of host immune response by virus

GO:0048298 positive regulation of isotype switching to IgA isotypes

GO:0050680 negative regulation of epithelial cell proliferation

GO:0050714 positive regulation of protein secretion

GO:0050921 positive regulation of chemotaxis

GO:0051781 positive regulation of cell division

GO:0051897 positive regulation of protein kinase B signaling cascade

GO:0060389 pathway-restricted SMAD protein phosphorylation

GO:0060391 positive regulation of SMAD protein import into nucleus

GO:0070723 response to cholesterol

AHP1734

154319 160659 161212

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