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PPAR Alpha antibody

Rabbit anti PPAR Alpha

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
PPAR Alpha

Product Code Applications Pack Size List Price Your Price Qty
AHP1459
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E P * WB 50 µg loader
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loader

Rabbit anti Mouse PPAR alpha antibody recognizes mouse Peroxisome proliferator-activated receptor alpha (PPAR ALPHA), a 52.2 kDa transcription factor belonging to the nuclear hormone receptor (NR1) family.

Peroxisome proliferator-activated receptors are vital in the regulation of cellular differentiation, development and metabolism. PPARA is also the target of hypolipidemic fibrates, a class of drugs used in cholesterol disorders.

PPARA alpha is expressed primarily in the liver, kidney, heart and skeletal muscle and is involved in the regulation of beta-oxidation of fatty acids.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Monkey
Chimpanzee
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to mouse PPAR ALPHA was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 1 to 18 of mouse PPAR alpha.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/8000
Immunohistology - Paraffin 1 20ug/ml
Western Blotting 1/500
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Mouse liver

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Synonyms
PPARA
RRID
AB_2252504
UniProt
P23204
Entrez Gene
Ppara
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003707 steroid hormone receptor activity
GO:0005634 nucleus
GO:0006006 glucose metabolic process
GO:0008289 lipid binding
GO:0006631 fatty acid metabolic process
GO:0008144 drug binding
GO:0008270 zinc ion binding
GO:0008544 epidermis development
GO:0016563 transcription activator activity
GO:0032099 negative regulation of appetite
GO:0042060 wound healing
GO:0043565 sequence-specific DNA binding
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0046321 positive regulation of fatty acid oxidation
GO:0046982 protein heterodimerization activity

AHP1459

158572 170123

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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