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GLI3 antibody

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AHP1456
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SDS Safety Datasheet SDS
E P * WB 50 µg loader
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Rabbit anti Human GLI3 antibody recognizes the Human Zinc Finger protein 3 (GLI-3), also known as GLI-Kruppel family member 3, a 169.8 kDa protein belonging to the GLI C2H2-type zinc finger protein of transcription factors.

GLI-3 and the related GLI-2 proteins are the primary transcription factors that mediate Sonic hedgehog (Shh) signals in the mouse, GLI-2 being primarily a transcriptional activator while GLI-3 is a transcriptional repressor. GLI-3 plays a role in the development of the limbs and brain. It is expressed in a wide range of adult tissues including the colon, lung, spleen, testis and placenta.

Defects in GLI3 are causative of a range of disease states including Pallister-Hall syndrome, type IV preaxial polydactyly, acrocallosal syndrome (ACS) and Greig cephalo-poly-syndactyly syndrome. GLI proteins are also implicated as potent oncogenes in the embryonal carcinoma cell.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chicken
Rat
Amphibia Expected from Sequence
Dog
Monkey
Mouse
Chimpanzee
Quail
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human GLI3 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acid sequence 41-57 of human Gli3 protein.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/6000
Immunohistology - Paraffin 1 1.25ug/ml
Western Blotting 1/500
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human brain, glioblastoma

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RRID
AB_2111920
UniProt
P10071
Entrez Gene
GLI3
GO Terms
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0005829 cytosol
GO:0005730 nucleolus
GO:0005929 cilium
GO:0008013 beta-catenin binding
GO:0008270 zinc ion binding
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0016564 transcription repressor activity
GO:0035035 histone acetyltransferase binding
GO:0042826 histone deacetylase binding
GO:0045060 negative thymic T cell selection
GO:0045879 negative regulation of smoothened signaling pathway
GO:0046638 positive regulation of alpha-beta T cell differentiation
GO:0046639 negative regulation of alpha-beta T cell differentiation
GO:0070242 thymocyte apoptosis
GO:0090090 negative regulation of canonical Wnt receptor signaling pathway

AHP1456

130715

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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