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CTGF antibody

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Anti Human CTGF Antibody thumbnail image 1 Anti Human CTGF Antibody thumbnail image 2 Anti Human CTGF Antibody thumbnail image 3
Anti Human CTGF Antibody gallery image 1
Formalin fixed, paraffin embedded human breast invasive malignant carcinoma stained with Rabbit anti Human CTGF (AHP1278) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
Anti Human CTGF Antibody gallery image 2
Formalin fixed, paraffin embedded human breast invasive malignant carcinoma stained with Rabbit anti Human CTGF (AHP1278) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0)
Anti Human CTGF Antibody gallery image 3
Published customer image:
Rabbit anti HumAN ctgf ANTIBODY (AHP1278) used to evaluate CTGF expression in rat adrenal glands by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
CTGF expression in adrenal glands 400× magnified in the control (A), experimental (B) and reversibility control (C) groups. Visible insular and irregular expression. IHC staining (bar = 50μm).

From: Zarobkiewicz MK, Wożniakowski MM, Wawryk-Gawda E, Sławiński MA, Halczuk P, Korolczuk A, Jodłowska-Jędrych B.
Decrease in Lipid Droplets in Adrenal Cortex of Male Wistar Rats after Chronic Exposure to Energy Drinks.
Medicina (Kaunas). 2018 Nov 19;54(5):90.
doi: 10.3390/medicina54050090.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rabbit anti Human CTGF:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CTGF

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Product Code Applications Pack Size List Price Your Price Qty
AHP1278B
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Rabbit anti Human CTGF antibody recognizes human CTGF (connective tissue growth factor), a 323 amino acid ~38 kDa polypeptide member of the CCN family of secreted cysteine rich regulatory proteins. CTGF is produced by vascular endothelial and umbilical vein cells, and interacts with several other growth factors, including transforming growth factor beta (TGF-beta), vascular endothelial growth factor (VEGF) and bone morphogenetic proteins (BMPs).

CTGF is a heparin-binding pleiotropic growth factor involved in chondrogenesis and potentially skeletogenesis. CTGF promotes the adhesion of epithelial cells and fibroblasts, stimulates integrin expression, and also promotes endothelial cell survival, migration and adhesion, implicating CTGF in angiogenesis. CTGF induces extracellular matrix formation, including the deposition of excessive collagen in fibrotic diseases, and has also been implicated in wound healing.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilized
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antisera to human CTGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 0.1mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Immunohistology - Paraffin
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated human CTGF antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with our purified human CTGF antibody (AHP1278) as the capture reagent and recombinant human CTGF (PHP174) as the standard.

References for CTGF antibody

  1. Westermann, D. et al. (2009) Gene deletion of the kinin receptor B1 attenuates cardiac inflammation and fibrosis during the development of experimental diabetic cardiomyopathy.
    Diabetes. 58: 1373-81.
  2. Waddell, J.M. et al. (2011) CTGF expression is up-regulated by PROK1 in early pregnancy and influences HTR-8/Svneo cell adhesion and network formation.
    Hum Reprod. 26 (1): 67-75.
  3. Vasilieva. O.V. et al. (2016) Connective tissue growth factor (CTGF) in the human dermis through ontogenesis
    Russian Journal of Developmental Biology. 47 (2): 63-68.
  4. Zarobkiewicz, M.K. et al. (2018) Decrease in Lipid Droplets in Adrenal Cortex of Male Wistar Rats after Chronic Exposure to Energy Drinks.
    Medicina (Kaunas). 54 (5):90.

Further Reading

  1. Moussad, E.E. & Brigstock, D.R. (2000) Connective tissue growth factor: what's in a name?
    Mol Genet Metab. 71 (1-2): 276-92.

Immunofluorescence

Synonyms
Connective Tissue Growth Factor
RRID
AB_2087369
UniProt
P29279
Entrez Gene
CTGF
GO Terms
GO:0005886 plasma membrane
GO:0006260 DNA replication
GO:0001558 regulation of cell growth
GO:0005520 insulin-like growth factor binding
GO:0005578 proteinaceous extracellular matrix
GO:0008201 heparin binding
GO:0008544 epidermis development
GO:0009611 response to wounding
GO:0044255 cellular lipid metabolic process

AHP1278B

165612

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