IL-5 antibody
Rabbit anti Human Interleukin-5:Biotin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-5
Antibody Quality and Performance Guarantee
| Rabbit anti Human Interleukin-5 antibody recognizes human IL-5 (Interleukin-5), a disulphide-linked homodimer consisting of two 115 amino acid chains, which is homologous to mouse eosinophil differentiation factor (EDF) and is produced by mast cells, eosinophils and T cells. IL-5 is a lineage-specific cytokine involved in the development and stimulation of eosinophils, and has been linked with diseases involving abnormally high levels of eosinophils, including parasitic infections and allergic airways eosinophilia, the major pathological symptom of allergic asthma. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Reconstitution
- Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antisera to human IL-5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-5.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.25 | 1.0ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- ELISA
- This antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with AHP1034 as the capture antibody.
Further Reading
-
Campbell, H.D. et al. (1987) Molecular cloning, nucleotide sequence, and expression of the gene encoding human eosinophil differentiation factor (interleukin 5).
Proc Natl Acad Sci U S A. 84 (19): 6629-33. -
Wang, J. et al. (1998) Circulating, but not local lung, IL-5 is required for the development of antigen-induced airways eosinophilia.
J Clin Invest. 102 (6): 1132-41.
- RRID
- AB_2249204
- UniProt
- P05113
- Entrez Gene
- IL5
- GO Terms
- GO:0005125 cytokine activity
- GO:0005137 interleukin-5 receptor binding
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
AHP1034B
147366
153540
159070
164816
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with IL-5 specificity
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