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IgM antibody

Goat anti Dog IgM:Alk. Phos.

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgM

Product Code Applications Pack Size List Price Your Price Qty
AAI30AB
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E 0.5 mg loader
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Goat anti Dog IgM polyclonal antibody recognizes canine IgM and shows no cross - reactivity with other canine immunoglobulin classes in immunoelectrophoresis and ELISA. Goat anti Dog IgM may cross react with IgM from other species.

Target Species
Dog
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Antiserum Preparation
Antisera to canine IgM were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Immunogen
Purified canine IgM.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC.
DO NOT FREEZE.

This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/10000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

References for IgM antibody

  1. Peters, I.R. et al. (2004) Measurement of immunoglobulin concentrations in the feces of healthy dogs.
    Clin Diagn Lab Immunol. 11 (5): 841-8.
  2. Carli, E. et al. (2009) Detection of erythrocyte binding IgM and IgG by flow cytometry in sick dogs with Babesia canis canis or Babesia canis vogeli infection.
    Vet Parasitol. 162: 51-7.
  3. Shin, N. et al. (2018) INCB040093 Is a Novel PI3Kδ Inhibitor for the Treatment of B Cell Lymphoid Malignancies.
    J Pharmacol Exp Ther. 364 (1): 120-30.
  4. Sfacteria, A. et al. (2021) Immune Cells and Immunoglobulin Expression in the Mammary Gland Tumors of Dog.
    Animals (Basel). 11(5):1189.
  5. Wysmołek, M.E. et al. (2022) Canine antibody response against Dirofilaria repens. in natural occult and microfilaremic infections
    Comparative Immunology, Microbiology and Infectious Diseases. : 101818.

ELISA

Western Blotting

RRID
AB_10844345
UniProt
P01874
GO Terms
GO:0003823 antigen binding

AAI30AB

158635 180108

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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