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IgG2

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5225-3004
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Native Human IgG2 is a purified preparation of human IgG2 obtained from human myeloma, serum or urine. A single line is observed by IEP with anti whole serum and anti human IgG2 antibodies. No reactivity is seen with anti Human IgG1, IgG3 or IgG4.

Target Species
Human
Product Form
Purified protein - liquid
Preparation
Prepared from human myeloma serum by ion exchange chromatography, gel filtration and protein A chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
0.1% EACA
0.01% Benzamidine
Approx. Protein Concentrations
1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

References for IgG2

  1. Haberland, A. et al. (2016) Aptamer BC 007 – a broad spectrum neutralizer of pathogenic autoantibodies against G-protein-coupled receptors.
    Eur J Pharmacol. Jul 1 [Epub ahead of print]

RRID
AB_615613
UniProt
P01859
Entrez Gene
IGHG2
GO Terms
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0005624 membrane fraction
GO:0006955 immune response

5225-3004

149164

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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