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IgG antibody

Goat anti Mouse IgG (H/L):FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(H/L)

Product Code Applications Pack Size List Price Your Price Qty
5178-2404
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Goat anti Mouse IgG antibody recognizes the heavy and light chains of mouse IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, mouse IgG and mouse serum.

Target Species
Mouse
Product Form
Purified Ig conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilized
Reconstitution
Use 1.0 ml of DI water. For extended storage, reconstitute with 50% glycerol instead of water and then aliquot contents at -20ºC.
Preparation
Purified Ig prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10 mg/ml Bovine Serum Albumin and 0.01% sodium azide
Immunogen
Native, whole IgG
Approx. Protein Concentrations
IgG concentration 2.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/2K 1/10K
Immunofluorescence 1/500 1/2500
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

References for IgG antibody

  1. Olimpio, R.M.C. et al. (2018) Cell viability assessed in a reproducible model of human osteoblasts derived from human adipose-derived stem cells.
    PLoS One. 13 (4): e0194847.

Further Reading

  1. The, T.H. & Feltkamp, T.E. (1970) Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation.
    Immunology. 18 (6): 865-73.

RRID
AB_616780
UniProt
P01868
P01869
P01843
P01845
P01867
P01865
P01863
P01864
P03987
P01844
P01837
Entrez Gene
Igk-C
LOC433053
Iglc2
Iglc3
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
AI324046
GO Terms
GO:0003823 antigen binding
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis

5178-2404

153989 170410

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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