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IgG antibody

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403005
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SDS Safety Datasheet SDS
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Goat anti Rabbit IgG antibody recognizes the heavy chain of rabbit IgG.

Cross-reactivity against rabbit IgM is reduced by solid phase adsorption. This antibody may react with IgG from other species.

Target Species
Rabbit
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to rabbit IgG were raised by repeated immunisations of goats with normal rabbit IgG.
The Ig fraction was purified by affinity chromatography on pooled rabbit IgG covalently linked to agarose.
The preparation has been cross absorbed against rabbit IgM to remove any potential cross reactivity.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
50% glycerol
Immunogen
Purified rabbit IgG from normal rabbit serum
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/8000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for IgG antibody

  1. Wegmann, F. et al. (2011) A Novel Strategy for Inducing Enhanced Mucosal HIV-1 Antibody Responses in an Anti-Inflammatory Environment
    PLoS One. 6(1):e15861.
  2. Radomirovic, M. et al. (2022) Phycocyanobilin-modified β-lactoglobulin exhibits increased antioxidant properties and stability to digestion and heating
    Food Hydrocolloids. 123: 107169.
  3. Mladenovic Stokanic, M. et al. (2023) Sandwich ELISA for the Quantification of Nucleocapsid Protein of SARS-CoV-2 Based on Polyclonal Antibodies from Two Different Species
    Int J Mol Sci. 25 (1): 333.

ELISA

RRID
AB_609686
UniProt
P01870
Entrez Gene
LOC100009097
GO Terms
GO:0003823 antigen binding

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rabbit Products
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