Collagen I antibody
Rabbit anti Mouse Collagen I antibody (2150-1410) used to demonstrate collagen I expressing fibroblastic reticular cells (FRCs) in vitro by immunofluorescence.
Image caption:
Confocal images of FRCs treated for 4 days with CCM, B16.F10 TCM, Veh– H2O, or 15 mmol/L LA and stained for collagen I (red) and nuclei (blue; left) and quantification thereof (right). n=2 independent experiments each with 8 fields of view analyzed. Scale bar: 50μm. Data are mean with SEM. Significance (, P <0.05; , P <0.01; , P <0.001; and , P <0.0001) was determined by unpaired two-tailed t test. B16, B16.F10.
From: Angela Riedel, Moutaz Helal, Luisa Pedro, Jonathan J. Swietlik, David Shorthouse, Werner Schmitz, Lisa Haas, Timothy Young, Ana S.H. da Costa, Sarah Davidson, Pranjali Bhandare, Elmar Wolf, Benjamin A. Hall, Christian Frezza, Thordur Oskarsson, Jacqueline D. Shields;
Tumor-Derived Lactic Acid Modulates Activation and Metabolic Status of Draining Lymph Node Stroma.
Cancer Immunology Research. 10 (4): 482-97;
doi: 10.1158/2326-6066.CIR-21-0778
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rabbit anti Mouse Collagen I
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Collagen I
Antibody Quality and Performance Guarantee
| Rabbit anti Mouse Collagen I antibody recognizes murine collagen 1. Collagen 1 is a fibrillar forming collagen, a secreted protein forming part of the extracellular matrix. Less than 1% cross reactivity has been observed with mouse collagen, types II, VI and III and human, chicken and rat collagen, type I. |
- Target Species
- Mouse
- Product Form
- Purified Ig - liquid
- Preparation
- Purified Ig prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
0.1M Citrate - Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
Antibiotic antimycotic mixture 1% - Immunogen
- Native type I collagen from mouse skin.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/100 | 1/400 |
| Immunofluorescence | |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
1/250 | 1/1000 |
| Radioimmunoassays | |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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References for Collagen I antibody
-
Uezumi, A. et al. (2011) Fibrosis and adipogenesis originate from a common mesenchymal progenitor in skeletal muscle.
J Cell Sci. 124: 3654-64. -
Takase, H.M. et al. (2013) FGF7 is a functional niche signal required for stimulation of adult liver progenitor cells that support liver regeneration.
Genes Dev. 27: 169-81. -
Birbrair A et al. (2013) Type-1 pericytes participate in fibrous tissue deposition in aged skeletal muscle.
Am J Physiol Cell Physiol. 305 (11): C1098-113. -
Randles, M.J. et al. (2015) Genetic Background is a Key Determinant of Glomerular Extracellular Matrix Composition and Organization.
J Am Soc Nephrol. 26 (12): 3021-34. -
Ma, M.S. et al. (2017) Characterization and comparison of osteoblasts derived from mouse embryonic stem cells and induced pluripotent stem cells.
J Bone Miner Metab. 35 (1): 21-30. -
Valero-Muñoz, V. et al. (2019) Supplementation with an insoluble fiber obtained from carob pod (Ceratonia siliqua L.) rich in polyphenols prevents dyslipidemia in rabbits through SIRT1/PGC-1α pathway.
Eur J Nutr. 58 (1): 357-66. -
Riedel, A. et al. (2022) Tumor-Derived Lactic Acid Modulates Activation and Metabolic Status of Draining Lymph Node Stroma
Cancer Immunology Research. 10 (4): 482-97.
- RRID
- AB_620433
- UniProt
- P11087
- Q01149
- Entrez Gene
- Col1a1
- Col1a2
- GO Terms
- GO:0001568 blood vessel development
- GO:0001649 osteoblast differentiation
- GO:0001957 intramembranous ossification
- GO:0001958 endochondral ossification
- GO:0005201 extracellular matrix structural constituent
- GO:0005584 collagen type I
- GO:0005737 cytoplasm
- GO:0010812 negative regulation of cell-substrate adhesion
- GO:0015031 protein transport
- View More GO Terms
- GO:0060325 face morphogenesis
- GO:0060346 bone trabecula formation
- GO:0060351 cartilage development involved in endochondral bone morphogenesis
- GO:0070208 protein heterotrimerization
- GO:0046332 SMAD binding
View more products with COLLAGEN specificity
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