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Collagen IV

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Native Human Collagen IV

Product Type
Purified Protein
Specificity
Collagen IV

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2150-0150
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Human collagen type IV derived from placenta.

Cross reactivity:
Human collagen type I-III, V, VI (<5%).
Non collagen proteins (<0.5%).

Target Species
Human
Product Form
Purified protein - liquid
Preparation
Salt and alcohol precipitations, DEAE chromatography. Purity and retention of native helical structure was controlled by SDS-PAGE, ORD measurement, ability to form microfibrils and by reaction with anti-collagen type specific monoclonal antibodies.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Purity
>95% by SDS-PAGE
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml
Protein Molecular Weight
340 kDa
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Further Reading

  1. Glanville, R.W. et al. (1979) Isolation and characterization of a native placental basement-membrane collagen and its component alpha chains.
    Eur J Biochem. 95 (2): 383-9.
  2. Sage, H. & Bornstein, P. (1979) Characterization of a novel collagen chain in human placenta and its relation to AB collagen.
    Biochemistry. 18 (17): 3815-22.
  3. Klasson, S.C. et al. (1986) The effects of tissue pretreatment and pepsin levels on the isolation of collagens from human placenta.
    Coll Relat Res. 6 (5): 397-408.
  4. Bornstein, P. & Sage, H. (1980) Structurally distinct collagen types.
    Annu Rev Biochem. 49: 957-1003.

UniProt
P02462
P08572
Q01955
P53420
P29400
Q14031
Entrez Gene
COL4A1
COL4A2
COL4A5
COL4A4
COL4A3
COL4A6
GO Terms
GO:0001525 angiogenesis
GO:0005201 extracellular matrix structural constituent
GO:0007411 axon guidance
GO:0048407 platelet-derived growth factor binding
GO:0005515 protein binding
GO:0005587 collagen type IV
GO:0016525 negative regulation of angiogenesis
GO:0030198 extracellular matrix organization
GO:0005605 basal lamina
GO:0032836 glomerular basement membrane development

2150-0150

130314 157149 158969 161621

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