IgA antibody
Biotinylated Goat anti Human IgA antibody used for the evaluation of human IgA responses to pneumococcal challenge by ELISA with subsequent detection by alkaline phosphatase conjugated streptavidin (STAR6B) and p-nitrophenyl phosphate.
Image caption:
IgG and IgA responses to whole cell 23F or 6B pneumococci following 23F or 6B challenge, respectively. ELISAs were performed using 23F or 6B pneumococci as targets to measure specific IgG (A, C and E) and IgA (B, D and F) titers in serum (A and B), NW (C and D) and BAL (E and F). Values shown are the mean antibody titers (of triplicates), pre and post 23F (n = 7) or 6B (n = 8) challenge (x-axis). *represents statistical significance between pre- and post-inoculation antibody titers.
From: Wright AKA, Ferreira DM, Gritzfeld JF, Wright AD, Armitage K, Jambo KC, et al.. (2012)
Human Nasal Challenge with Streptococcus pneumoniae Is Immunising in the Absence of Carriage.
PLoS Pathog 8(4): e1002622.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Biotin conjugated Goat anti Human IgA antibody (205008) used to evaluate virus specific IgA responses in human samples by ELISA.
Image caption:
Recovery of influenza specific nasal IgA is not affected by posting samples. Adult volunteers, had nasal samples collected by flocked swab. Samples were either processed directly from the nose or posted via the internal mail system. Having processed the samples they were analysed for H1N1 (a) or H3N2 (b) specific nasal IgA by ELISA. n = 6.
From: Tregoning JS.
Differences in nasal immunoglobulin A responses to influenza vaccine strains after live attenuated influenza vaccine (LAIV) immunization in children.
Clin Exp Immunol. 2020 Feb;199 (2):109-18.
doi: 10.1111/cei.13395.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Goat anti Human IgA:RPE
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgA
Antibody Quality and Performance Guarantee
| Goat anti Human IgA antibody recognizes the heavy chain of human IgA as demonstrated by ELISA and Flow cytometry. IgA is the major catagory of antibody present in mucosal secretions and acts as a first line of defense against inhaled or ingested pathogens at the mucosal interface. IgA is also present in serum where it acts to eliminate pathogens via interaction with FcαR, also known as CD89. (Woof et al. 2004). Biotinylated Goat anti Human IgA antibody (205008) has been cross-adsorbed against human IgD, IgG and IgM to ensure class specificity. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on pooled human IgA
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose) - Immunogen
- Human IgA.
- Approx. Protein Concentrations
- 0.5 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
+4ºC DO NOT FREEZE
This product is photosensitive and should be protected from light.
This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Human Seroblock | BUF070A | F | 50 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
| Human Seroblock | BUF070B | F | 200 Test | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Human Seroblock | ||||||
References for IgA antibody
-
Wright, A.K. et al. (2012) Human Nasal Challenge with Streptococcus pneumoniae Is Immunising in the Absence of Carriage.
PLoS Pathog. 8: e1002622. -
Pennington, S.H. et al. (2016) Polysaccharide-Specific Memory B Cells Predict Protection against Experimental Human Pneumococcal Carriage.
Am J Respir Crit Care Med. 194 (12): 1523-31. -
Turner, P.J. et al. (2020) Differences in nasal immunoglobulin A responses to influenza vaccine strains after live attenuated influenza vaccine (LAIV) immunization in children.
Clin Exp Immunol. 199 (2): 109-118. -
Cole, M.E. et al. (2021) Pre-existing influenza-specific nasal IgA or nasal viral infection does not affect live attenuated influenza vaccine immunogenicity in children.
Clin Exp Immunol. 204 (1): 125-133.
- RRID
- AB_616850
- UniProt
- P01876
- P01877
- Entrez Gene
- IGHA1
- IGHA2
- GO Terms
- GO:0005515 protein binding
- GO:0003823 antigen binding
- GO:0006955 immune response
- GO:0018298 protein-chromophore linkage
- GO:0005624 membrane fraction
View more products with IgA specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up