Lambda Light Chain antibody
Goat anti Mouse lambda light chain antibody (106001) used as a coating reagent in ELISA for determination of mouse antibody levels in serum samples.
Image caption:
Inflammation after immunization with unmodified mRNA is associated with dampened adaptive immune responses.
Blood was collected for analysis of HA specific antibody 21 days (B) and 42 days (C) after study start; 42 days after the study, spleens were collected and assessed for HA specific T cells by ELISPOT (D).
From: Wang Z, Jacobus EJ, Stirling DC, Krumm S, Flight KE, Cunliffe RF, Mottl J, Singh C, Mosscrop LG, Santiago LA, Vogel AB, Kariko K, Sahin U, Erbar S, Tregoning JS.
Reducing cell intrinsic immunity to mRNA vaccine alters adaptive immune responses in mice.
Mol Ther Nucleic Acids. 2023 Oct 5;34:102045.
doi: 10.1016/j.omtn.2023.102045.
This image is from an open access article distributed under terms of a Creative Commons Attribution License
Goat anti Mouse Lambda Light Chain:Alk. Phos.
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Lambda Light Chain
Antibody Quality and Performance Guarantee
| Goat anti Mouse Lambda Light Chain antibody recognizes mouse lambda 1 and lambda 2 light chains (free and bound) as demonstrated by ELISA. To minimise cross reactivity this product has been cross absorbed against mouse paraproteins with kappa light chains. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Antiserum Preparation
- Antisera to mouse lambda light chains were raised by repeated immunisations of goats with highly purified antigen. Purified Ig was prepared from whole serum by affinity chromatography on pooled mouse Igs with lambda chains covalently linked to agarose.
- Buffer Solution
- TRIS buffered saline, 1mM MgCl2
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
50% Glycerol - Immunogen
- Mouse paraproteins with lambda 1 and lambda 2 light chains.
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/2000 | 1/4000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Lambda Light Chain antibody
-
Rops, A.L. et al. (2010) The tetraspanin CD37 protects against glomerular IgA deposition and renal pathology.
Am J Pathol. 176 (5): 2188-97. -
Lewis, A. et al. (2017) Recovery from desensitization of IgE-dependent responses in human lung mast cells.
Clin Exp Allergy. 47 (8): 1022-31. -
Blakney, A.K. et al. (2021) Innate Inhibiting Proteins Enhance Expression and Immunogenicity of Self-Amplifying RNA.
Mol Ther. 29 (3): 1174-85. -
Wang, Z. et al. (2023) Reducing cell intrinsic immunity to mRNA vaccine alters adaptive immune responses in mice.
Mol Ther Nucleic Acids. 34: 102045.
- RRID
- AB_609764
- UniProt
- P01843
- P01844
- Entrez Gene
- LOC433053
- Iglc2
- GO Terms
- GO:0003823 antigen binding
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
106004
156759
157828
163259
167950
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with LAMBDA LIGHT CHAIN specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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