Kappa Light Chain antibody
Goat anti Mouse kappa light chain antibody (105001) used as a coating reagent in ELISA for determination of mouse antibody levels in serum samples.
Image caption:
Inflammation after immunization with unmodified mRNA is associated with dampened adaptive immune responses.
Blood was collected for analysis of HA specific antibody 21 days (B) and 42 days (C) after study start; 42 days after the study, spleens were collected and assessed for HA specific T cells by ELISPOT (D).
From: Wang Z, Jacobus EJ, Stirling DC, Krumm S, Flight KE, Cunliffe RF, Mottl J, Singh C, Mosscrop LG, Santiago LA, Vogel AB, Kariko K, Sahin U, Erbar S, Tregoning JS.
Reducing cell intrinsic immunity to mRNA vaccine alters adaptive immune responses in mice.
Mol Ther Nucleic Acids. 2023 Oct 5;34:102045.
doi: 10.1016/j.omtn.2023.102045.
This image is from an open access article distributed under terms of a Creative Commons Attribution License
Goat anti Mouse Kappa Light Chain:Alk. Phos.
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Kappa Light Chain
Antibody Quality and Performance Guarantee
| Goat anti mouse kappa light chain polyclonal antibody is specific for mouse kappa light chains (free and bound). This antibody is cross-adsorbed against mouse lambda light chains. |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on kappa light chains linked to agarose
- Buffer Solution
- TRIS buffered saline, 1mM MgCl2
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
50% Glycerol - Immunogen
- Mouse paraproteins with kappa light chains.
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/2000 | 1/4000 |
| Immunoblotting | |
||
| Immunohistology - Frozen | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Kappa Light Chain antibody
-
Blakney, A.K. et al. (2021) Innate Inhibiting Proteins Enhance Expression and Immunogenicity of Self-Amplifying RNA.
Mol Ther. 29 (3): 1174-85. -
Wang, Z. et al. (2023) Reducing cell intrinsic immunity to mRNA vaccine alters adaptive immune responses in mice.
Mol Ther Nucleic Acids. 34: 102045.
- RRID
- AB_619830
- UniProt
- P01837
- Entrez Gene
- Igk-C
- GO Terms
- GO:0003823 antigen binding
105004
160575
220414
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with KAPPA LIGHT CHAIN specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Mouse ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up