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IgG IgA IgM antibody

Goat anti Mouse IgG/A/M (H/L):Texas Red®

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG IgA IgM
Region
(H/L)

Product Code Applications Pack Size List Price Your Price Qty
101007
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Goat anti Mouse IgG/A/M reacts with the heavy and light chains of all major classes of mouse immunoglobulin.

The antibody has been adsorbed against human serum to minimise cross-reactivity with human immunoglobulins.

Target Species
Mouse
Product Form
Purified IgG conjugated to Texas Red - liquid
Preparation
Purified IgG prepared by affinity chromatography on pooled mouse IgM, IgG and IgA covalently linked to agarose
Antiserum Preparation
Antisera to Mouse IgG, IgA and IgM were raised by repeated immunisation of goats with pooled mouse IgG, IgA and IgM paraproteins.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Purified mouse IgG, IgA and IgM.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Texas Red 596 615
Regulatory
For research purposes only
Guarantee
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
Acknowledgements
Texas Red is a trademark of Molecular Probes, Inc.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/20
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for IgG IgA IgM antibody

  1. Ding, G. et al. (2010) Effect of cryopreservation on biological and immunological properties of stem cells from apical papilla.
    J Cell Physiol. 22: 415-22.
  2. Shao, L. et al. (2015) Comparative In Vitro and In Vivo Studies of Porcine Rotavirus G9P[13] and Human Rotavirus Wa G1P[8].
    J Virol. 90 (1): 142-51.
  3. Iver, S. et al. (2019) Probing BAK and BAX Activation and Pore Assembly with Cytochrome c Release, Limited Proteolysis, and Oxidant-Induced Linkage.
    Methods Mol Biol. 1877: 201-16.

RRID
AB_609678
UniProt
P01864
P01872
P03987
P01873
P01868
P01865
P01869
P01878
P01867
P01863
P01844
P01843
P01845
P01834
Entrez Gene
IGKC
LOC433053
Iglc2
Iglc3
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
Ighm
Ighm
Igh-VJ558
AI324046
GO Terms
GO:0005515 protein binding
GO:0003823 antigen binding
GO:0005576 extracellular region
GO:0006958 complement activation, classical pathway
GO:0045087 innate immune response
GO:0009897 external side of plasma membrane
GO:0019815 B cell receptor complex
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0050766 positive regulation of phagocytosis
GO:0000187 activation of MAPK activity
GO:0004888 transmembrane receptor activity
GO:0019882 antigen processing and presentation
GO:0030890 positive regulation of B cell proliferation
GO:0043410 positive regulation of MAPKKK cascade
GO:0045022 early endosome to late endosome transport
GO:0045807 positive regulation of endocytosis
GO:0048471 perinuclear region of cytoplasm
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050853 B cell receptor signaling pathway

101007

156248 156518 158472

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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