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Angiotensin I antibody | Ang E9 (BGN/KA/4H)

Mouse anti Human Angiotensin I

Product Type
Monoclonal Antibody
Clone
Ang E9 (BGN/KA/4H)
Isotype
IgG2a
Specificity
Angiotensin I

Product Code Applications Pack Size List Price Your Price Qty
0500-0013
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.2 mg loader
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loader

Mouse anti Human Angiotensin I, clone Ang E9, is specific for Angiotensin I and does not react with angiotensin II, III or angiotensinogen in ELISA.

Angiotensin I is important in the Renin-Angiotensin-Aldosterone System (RAAS). RAAS is responsible for the regulation of blood pressure and water retention in the body.

Angiotensin I is produced from a reaction between the renal enzyme Renin and angiotensinogen. It is converted by Angiotensin Converting Enzyme (ACE) into a vasoactive form called Angiotensin II which is responsible for increasing blood pressure.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide, PFHLVIHNES(C). corresponding to amino acids 40-49 of the angiotensinogen precursor conjugated to HMW carrier protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
ELISA
In a simple indirect ELISA, using 1.0 µg of Angiotensin I coated per well, the 50% binding was observed at an antibody dilution of 1/12K. Under the same conditions, the end point titre was observed at an antibody dilution of 1/100K.

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RRID
AB_617189
UniProt
P01019
Entrez Gene
AGT
GO Terms
GO:0001819 positive regulation of cytokine production
GO:0001822 kidney development
GO:0001974 blood vessel remodeling
GO:0002018 renin-angiotensin regulation of aldosterone production
GO:0002019 regulation of renal output by angiotensin
GO:0003078 regulation of natriuresis
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0005625 soluble fraction
GO:0006800 oxygen and reactive oxygen species metabolic process
GO:0007199 G-protein signaling, coupled to cGMP nucleotide second messenger
GO:0007200 activation of phospholipase C activity by G-protein coupled receptor protein signaling pathway coupled to IP3 second messenger
GO:0007263 nitric oxide mediated signal transduction
GO:0007267 cell-cell signaling
GO:0008083 growth factor activity
GO:0010595 positive regulation of endothelial cell migration
GO:0010613 positive regulation of cardiac muscle hypertrophy
GO:0010698 acetyltransferase activator activity
GO:0010744 positive regulation of macrophage derived foam cell differentiation
GO:0010873 positive regulation of cholesterol esterification
GO:0014068 positive regulation of phosphatidylinositol 3-kinase cascade
GO:0014873 response to muscle activity involved in regulation of muscle adaptation
GO:0019229 regulation of vasoconstriction
GO:0031702 type 1 angiotensin receptor binding
GO:0031703 type 2 angiotensin receptor binding
GO:0033864 positive regulation of NAD(P)H oxidase activity
GO:0034374 low-density lipoprotein particle remodeling
GO:0043065 positive regulation of apoptosis
GO:0044255 cellular lipid metabolic process
GO:0045742 positive regulation of epidermal growth factor receptor signaling pathway
GO:0048146 positive regulation of fibroblast proliferation
GO:0050729 positive regulation of inflammatory response
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051387 negative regulation of nerve growth factor receptor signaling pathway

0500-0013

160729 170208

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