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ACTH antibody

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Rabbit anti Human ACTH

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
ACTH

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Product Code Applications Pack Size List Price Your Price Qty
0178-0204
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E IF P 0.5 ml loader
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loader

Rabbit anti Human ACTH antibody recognizes human adrenocorticotrophic hormone, also known as corticotrophin. ACTH is a 4.5 kDa hormone derived from cleavage of pro-opiomelanocortin (POMC), produced in the pituitary gland in response to corticotrophin releasing hormone (CRH) activity and functions to stimulate release of adrenal cortisol.

Rabbit anti Human ACTH polyclonal antibody is directed against the N-terminal portion of adrenocorticotrophic hormone and also recognizes the ACTH cleavage fragment α-melanocyte stimulating hormone (α-MSH), responsible for melanin pigmentation control in most vertebrate species (Fiordelisio et al. 2006). Rabbit anti Human ACTH does not recognize the C-terminal ACTH cleavage product, corticotropin-like intermediary peptide (CLIP).

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Broad Expected from Sequence
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified Ig - liquid
Preparation
Purified Ig prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to the amino acids 1-24 (SYSMEHFRWGKPVGKKRRPVKVYP) of ACTH conjugated to Keyhole limpet Haemocyanin.
Approx. Protein Concentrations
5.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/1000
Immunofluorescence
Immunohistology - Frozen 1/100 1/200
Immunohistology - Paraffin 1/100 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
ACTH-containing cells of the anterior pituitary gland.

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References for ACTH antibody

  1. Osamura, R.Y. et al. (1980) Adrenocorticotropic hormone cells and immunoreactive beta-endorphin cells in the human pituitary gland: normal and pathologic conditions studied by the peroxidase-labeled antibody method.
    Am J Pathol. 99 (1): 105-24.
  2. Fiordelisio, T. et al. (2006) Cells of proopiomelanocortin lineage from the rodent anterior pituitary lack sexually dimorphic expression of neurofilaments.
    Neuroendocrinology. 83:360-70.
  3. Farris, G.M. et al. (2007) Recombinant rat and mouse growth hormones: risk assessment of carcinogenic potential in 2-year bioassays in rats and mice.
    Toxicol Sci. 97: 548-61.

RRID
AB_620352
UniProt
P01189
Entrez Gene
POMC
GO Terms
GO:0005179 hormone activity
GO:0005615 extracellular space
GO:0030141 stored secretory granule
GO:0006091 generation of precursor metabolites and energy
GO:0007218 neuropeptide signaling pathway
GO:0007267 cell-cell signaling
GO:0008217 regulation of blood pressure
GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
GO:0016486 peptide hormone processing
GO:0031781 type 3 melanocortin receptor binding
GO:0031782 type 4 melanocortin receptor binding
GO:0032098 regulation of appetite
GO:0032720 negative regulation of tumor necrosis factor production
GO:0033059 cellular pigmentation
GO:0034641 cellular nitrogen compound metabolic process
GO:0042446 hormone biosynthetic process
GO:0070996 type 1 melanocortin receptor binding

0178-0204

160926 166437 1705

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