Polyubiquitin Antibody | FK1

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Polyubiquitin Antibody | FK1 gallery image 1

Western blot of multi-ubiquitin chains using Mouse anti polyubiquitin (clone FK1, MCA6042) (lanes A-C) and Mouse anti mono- and molyubiquitin (clone FK2, MCA6035) (lanes D-F).
Lanes A and D: K48-linked chains. Lanes B and E: K29-linked chains. Lanes C and F: K63-linked chains

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  • Polyubiquitin Antibody | FK1 thumbnail image 1
  • Mouse anti Polyubiquitin
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  • Product Type
    Monoclonal Antibody
  • Clone
    FK1
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6042E, WBdatasheet pdfdatasheet pdf50 µg
    MCA6042
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti polyubiquitin antibody clone FK1 recognizes polyubiquitin chains.

      Ubiquitin, as the name implies, is a ubiquitously expressed and highly conserved protein of 8.6 kDa. The protein is covalently linked to selected lysine residues in a post-translational modification processes known as ubiquitylation or ubiquitination. This chemical reaction is mediated by three different protein families; ubiquitin-activating enzymes (also known as E1s), ubiquitin-conjugating enzymes (also known as E2s) and ubiquitin ligases (also known as E3s) (Hershko and Ciechanover 1998).

      The impact of ubiquitination depends on whether a single ubiquitin moiety (monoubiquitination) or an ubiquitin chain (polyubiquitination) has been attached to a protein. Monoubiquitination tends to trigger cellular processes related to endocytosis and membrane trafficking (Haglund et al. 2003) while the impact of polyubiquitination varies depending on how the ubiquitin residues in the chain have been linked. Attachment of Lysine-48 ubiquitin chains results in degradation by the 20S proteasome while addition of Lysine-63 ubiquitin chains mediates DNA damage and NFkappaB signaling (Chen 2005; Mocciaro and Rape 2012). Lysine-6, Lysine-11, Lysine-27, Lysine-29 and Lysine-33 chains have also been reported (Komander 2009; Ye and Rape 2011).

      When comparing staining of mouse anti polyubiquitin antibody (clone FK1) against staining with mouse anti mono or polyubiquitin antibody (clone FK2) or pan ubiquitin antibody (clone P4D1) one can determine, if a protein target is mono- or polyubiquitinated.
    • Intended Use
    • Target Species
      Broad
    • Product Form
      Purified IgM - liquid
    • Reconstitution
    • Preparation
      Purified IgM prepared by ion exchange chromatography from tissue culture supernatant
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
    • Immunogen
      Poly-ubiquitinylated lysozyme
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted.
    • Shelf Life
      12 months from date of despatch
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Western Blotting1/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Mouse anti polyubiquitin is specific for ubiquitin-protein conjugates and shows no reactivity with free ubiquitin. Use of milk based blocking reagents is not recommended. 1% BSA in PBS or TBS Tween should be used instead.
    • Instructions For Use

    Additional Polyubiquitin Antibody Formats

    Formats Clone Applications Sizes available
    Polyubiquitin Antibody : Purified FK1 E, WB 50 µg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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