HA-Tag antibody
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Rabbit anti HA-Tag
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- HA-Tag
Antibody Quality and Performance Guarantee
| Rabbit anti HA-Tag antibody recognizes the HA (Haemagglutinin) epitope tag peptide sequence YPYDVPDYA, when fused to either the amino- or carboxyl- terminus of target proteins, including expression in many frequently used expression vectors. Rabbit anti HA-Tag antibody has been tested against both the immunogen and recombinant proteins containing the HA sequence in ELISA and Western blotting. In Western blotting of bacterial extracts Rabbit anti HA-Tag antibody has been shown not to cross-react with any endogenous proteins. |
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Haemagglutinin epitope were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Keyhole limpet hemocyanin conjugated epitope tag peptide YPYDVPDYA (114-122) from haemagglutinin influenza. A cysteine residue was used to facilitate coupling at the C-terminal end.
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Immunohistology - Paraffin 1 | |
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| Western Blotting | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Further Reading
-
Field, J. et al. (1988) Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.
Mol Cell Biol. 8 (5): 2159-65. -
Wilson, I.A. et al. (1984) The structure of an antigenic determinant in a protein.
Cell. 37 (3): 767-78.
- Synonyms
- Hemagglutinin Tag
- RRID
- AB_808596
- UniProt
- Q03909
- GO Terms
- GO:0016021 integral to membrane
- GO:0019031 viral envelope
- GO:0019064 viral envelope fusion with host membrane
- GO:0020002 host cell plasma membrane
- GO:0046789 host cell surface receptor binding
- GO:0055036 virion membrane
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