Histone H4 Ac8 antibody




Product Details
- Target Species
- Synthetic Peptide
- Species Cross-Reactivity
-
Target Species Cross Reactivity Drosophila Yeast Mammals Amphibia Plants - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Serum - liquid
- Antiserum Preparation
- Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Ovalbumin-conjugated peptide: NSGRGKGGAcKGLGKYCc.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P62805
- Entrez Gene
- HIST2H4B
- GO Terms
- GO:0005515 protein binding
- GO:0000723 telomere maintenance
- GO:0000786 nucleosome
- GO:0003677 DNA binding
- GO:0005654 nucleoplasm
- GO:0048015 phosphatidylinositol-mediated signaling
- GO:0034080 CenH3-containing nucleosome assembly at centromere
- GO:0045653 negative regulation of megakaryocyte differentiation
- Regulatory
- For research purposes only
Applications of Histone H4 Ac8 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Chromatin Immunoprecipitation | |||
ELISA | 1/800 | ||
Immunofluorescence | 1/500 | ||
Immunoprecipitation | |||
Western Blotting | 1/1000 |
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E WB | 1 mg |
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Goat anti Rabbit IgG (Fc):FITC | STAR121F | F | 1 mg |
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|
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E WB | 1 mg |
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|
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C E WB | 1 mg |
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|
Sheep anti Rabbit IgG:FITC | STAR34B | C F | 1 mg |
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|
Sheep anti Rabbit IgG:RPE | STAR35A | F | 1 ml |
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Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | F IF WB | 0.1 mg | ||
Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | F IF WB | 0.1 mg |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
References for Histone H4 Ac8 antibody
-
Turner, B. M. et al. (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabelling with site-specific antibodies.
FEBS Letters 253: 141-145. -
Turner, B. M. et al. (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei.
Cell 69: 375-384. -
Belyaev, N. D. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.
Hum. Genet. 97: 573-578. -
Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 8: 1. -
Chen, W.Y. et al. (2000) Molecular mechanism for silencing virally transduced genes involves histone deacetylation and chromatin condensation.
Proc Natl Acad Sci U SA. 97: 377-82. -
Friis, R.M. et al. (2009) A glycolytic burst drives glucose induction of global histone acetylation by picNuA4 and SAGA.
Nucleic Acids Res. 37: 3969-80. -
Ito, K. et al. (2000) Glucocorticoid receptor recruitment of histone deacetylase 2 inhibits interleukin-1beta-induced histone H4 acetylation on lysines 8 and 12.
Mol Cell Biol. 20: 6891-903. -
Kobor, M.S. et al (2004) A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin.
PLoS Biol. 2: E131. -
Lin, X. et al. (2001) Reversal of GSTP1 CpG island hypermethylation and reactivation of pi-class glutathione S-transferase (GSTP1) expression in human prostate cancer cells by treatment with procainamide.
Cancer Res. 61: 8611-6. -
Nie, M. et al. (2003) Transcriptional regulation of cyclooxygenase 2 by bradykinin and interleukin-1beta in human airway smooth muscle cells: involvement of different promoter elements, transcription factors, and histone h4 acetylation.
Mol Cell Biol. 23: 9233-44. -
Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 8: 1. -
Torres-Machorro, A.L. et al. (2015) The Set3 Complex Antagonizes the MYST Acetyltransferase Esa1 in the DNA Damage Response.
Mol Cell Biol. 35 (21): 3714-25.
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