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Histone H4 Ac8 antibody

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Anti Histone H4 (Ac8) Antibody thumbnail image 1 Anti Histone H4 (Ac8) Antibody thumbnail image 2 Anti Histone H4 (Ac8) Antibody thumbnail image 3 Anti Histone H4 (Ac8) Antibody thumbnail image 4
Anti Histone H4 (Ac8) Antibody gallery image 1
Published customer image:
Rabbit anti Histone H4 (Ac8) antibody (AHP415) used for the evaluation of acetylated histone expression in yeast cell lysates by western blotting.
Image caption:
Eaf1p Was a Subunit of the NuA4 HAT (A) Eaf1-HA associated with NuA4 subunits. Immunoblots of analytical-scale TAP purifications are shown. The captured TAP-tagged protein is indicated above the gels, and the protein that was tested for association is indicated at the right. (B) Strains lacking EAF1 have defects in histone H4 acetylation. Whole cell extracts from mutant strains indicated on the top were tested for global histone acetylation using antibodies directed against different forms of acetylated H4 and H2A as indicated on the right.

From: Kobor MS, Venkatasubrahmanyam S, Meneghini MD, Gin JW, Jennings JL, Link AJ, et al. (2004)
A Protein Complex Containing the Conserved Swi2/Snf2-Related ATPase Swr1p Deposits Histone Variant H2A.Z into Euchromatin.
PLoS Biol 2(5): e131.
Anti Histone H4 (Ac8) Antibody gallery image 2
Published customer image:
Rabbit anti Histone H4 AcK8 antibody (AHP415) used for the assessment of histone H4 acetylation at lysine 8 in a rat model of colitis by western blotting.
Image caption:
Acetylation on histone 4 (H4) specific lysine residues 5 (K5) (A), 8 (K8) (B), 12 (K12) (C) and 16 (K16) (D) in a Sham (control) and trinitrobenzene sulfonic acid (TNBS) rat model of colitis. Results were obtained by Western blotting. In order to evaluate changes in density from different western blotting experiments control densitometry was denoted as 100% and differences were accounted as increase percentage of the control. Representative examples of bands obtained are also illustrated. Columns represent the densitometric evaluation of three independent experiments (mean &pluxmn; SEM). (*p <0.05 vs Sham proximal or Sham distal respectively).

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N.
Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan27;8(1):1.
Anti Histone H4 (Ac8) Antibody gallery image 3
Published customer image:
Rabbit anti Histone H4 AcK8 antibody (AHP415) used for the assessment of histone H4 acetylation at lysine 8 in a Lewis and Sprague Dawley rat model of DSS treatment by western blotting.
Image caption:
Acetylation on histone 4 (H4) specific lysine residues 5 (K5), 8 (K8), 12 (K12) and 16 (K16) in Lewis and Sprague-Dawley dextran sulfate sodium (5% DSS). A: Representative bands of H4K5, K8, K12 and K16 acetylation. Lanes for Lewis rats represent: non-DSS treated (control) and DSS-treated. Likewise representative bands are illustrated for the Sprague-Dawley rats. Graphical representation of Western blotting data. H4 acetylation of K5 (B), K8 (C), K12 (D) and K16 (E). Columns represent the mean ± SEM (bar) of three independent experiments.

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N.
Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan27;8(1):1.
Anti Histone H4 (Ac8) Antibody gallery image 4
Published customer image:
Rabbit anti Histone H4 AcK8 antibody (AHP415) used for the assessment of histone H4 acetylation at lysine 8 in Chron~s disease biopsy material lysates by western blotting.
Image caption:
Acetylation on histone 4 (H4) and H4 lysine residues in Crohn's disease. Columns represent the mean ± SEM of three independent experiments. Four biopsies were pooled to obtain sufficient protein for one experiment (50 μg of protein) (*p <0.05 vs control). Pan acetylation on H4 in Crohn's disease (A). Acetylation on histone 4 (H4) specific lysine residues 5 (K5) (B), K8 (C), K12 (D), and 16 (E), in non-inflamed, inflamed tissue and Peyer's patches of Crohn's disease patients. Results were obtained by Western blotting. Columns represent the mean ± SEM of three independent experiments. (*p <0.05 vs control, #p <0.005 vs non-inflamed CD). Representative images of the bands obtained are illustrated.

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N.
Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan27;8(1):1.

Rabbit anti Histone H4 (Ac8)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP415
Datasheet
Ch E IF IP WB
SDS
0.1 ml loader

Rabbit anti Histone 4(Ac8) antibody recognizes histone H4 only when acetylated at lysine 8.

Product Details

Target Species
Synthetic Peptide
Species Cross-Reactivity
Target SpeciesCross Reactivity
Drosophila
Yeast
Mammals Expected from Sequence
Amphibia Expected from Sequence
Plants Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Ovalbumin-conjugated peptide: NSGRGKGGAcKGLGKYCc.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P62805
Entrez Gene
HIST2H4B
GO Terms
GO:0005515 protein binding
GO:0000723 telomere maintenance
GO:0000786 nucleosome
GO:0003677 DNA binding
GO:0005654 nucleoplasm
GO:0048015 phosphatidylinositol-mediated signaling
GO:0034080 CenH3-containing nucleosome assembly at centromere
GO:0045653 negative regulation of megakaryocyte differentiation
Regulatory
For research purposes only

Applications of Histone H4 Ac8 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Chromatin Immunoprecipitation
ELISA 1/800
Immunofluorescence 1/500
Immunoprecipitation
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Application Based External Images

Chromatin Immunoprecipitation

Immunofluorescence

Western Blotting

Product Specific References

References for Histone H4 Ac8 antibody

  1. Turner, B. M. et al. (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabelling with site-specific antibodies.
    FEBS Letters 253: 141-145.
  2. Turner, B. M. et al. (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei.
    Cell 69: 375-384.
  3. Belyaev, N. D. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.
    Hum. Genet. 97: 573-578.
  4. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
    J Inflamm (Lond). 8: 1.
  5. Chen, W.Y. et al. (2000) Molecular mechanism for silencing virally transduced genes involves histone deacetylation and chromatin condensation.
    Proc Natl Acad Sci U SA. 97: 377-82.
  6. Friis, R.M. et al. (2009) A glycolytic burst drives glucose induction of global histone acetylation by picNuA4 and SAGA.
    Nucleic Acids Res. 37: 3969-80.
  7. Ito, K. et al. (2000) Glucocorticoid receptor recruitment of histone deacetylase 2 inhibits interleukin-1beta-induced histone H4 acetylation on lysines 8 and 12.
    Mol Cell Biol. 20: 6891-903.
  8. Kobor, M.S. et al (2004) A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin.
    PLoS Biol. 2: E131.
  9. Lin, X. et al. (2001) Reversal of GSTP1 CpG island hypermethylation and reactivation of pi-class glutathione S-transferase (GSTP1) expression in human prostate cancer cells by treatment with procainamide.
    Cancer Res. 61: 8611-6.
  10. Nie, M. et al. (2003) Transcriptional regulation of cyclooxygenase 2 by bradykinin and interleukin-1beta in human airway smooth muscle cells: involvement of different promoter elements, transcription factors, and histone h4 acetylation.
    Mol Cell Biol. 23: 9233-44.
  11. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
    J Inflamm (Lond). 8: 1.
  12. Torres-Machorro, A.L. et al. (2015) The Set3 Complex Antagonizes the MYST Acetyltransferase Esa1 in the DNA Damage Response.
    Mol Cell Biol. 35 (21): 3714-25.

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