Histone H4 Ac8 antibody

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Rabbit anti Histone H4 (Ac8)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP415 Ch E IF IP WB 0.1 ml
Rabbit anti Histone 4(Ac8) antibody recognizes histone H4 only when acetylated at lysine 8.

Product Details

Target Species
Synthetic Peptide
Species Cross-Reactivity
Target SpeciesCross Reactivity
Drosophila
Yeast
Mammals
Amphibia
Plants
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Ovalbumin-conjugated peptide: NSGRGKGGAcKGLGKYCc.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P62805 Related reagents
Entrez Gene
HIST2H4B Related reagents
GO Terms
GO:0005515 protein binding
GO:0000723 telomere maintenance
GO:0000786 nucleosome
GO:0003677 DNA binding
GO:0005654 nucleoplasm
GO:0048015 phosphatidylinositol-mediated signaling
GO:0034080 CenH3-containing nucleosome assembly at centromere
GO:0045653 negative regulation of megakaryocyte differentiation
Regulatory
For research purposes only

Applications of Histone H4 Ac8 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Chromatin Immunoprecipitation
ELISA 1/800
Immunofluorescence 1/500
Immunoprecipitation
Western Blotting 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Application Based External Images

Chromatin Immunoprecipitation

Immunofluorescence

Western Blotting

Product Specific References

References for Histone H4 Ac8 antibody

  1. Turner, B. M. et al. (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabelling with site-specific antibodies.
    FEBS Letters 253: 141-145.
  2. Turner, B. M. et al. (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei.
    Cell 69: 375-384.
  3. Belyaev, N. D. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.
    Hum. Genet. 97: 573-578.
  4. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
    J Inflamm (Lond). 8: 1.
  5. Chen, W.Y. et al. (2000) Molecular mechanism for silencing virally transduced genes involves histone deacetylation and chromatin condensation.
    Proc Natl Acad Sci U SA. 97: 377-82.
  6. Friis, R.M. et al. (2009) A glycolytic burst drives glucose induction of global histone acetylation by picNuA4 and SAGA.
    Nucleic Acids Res. 37: 3969-80.
  7. Ito, K. et al. (2000) Glucocorticoid receptor recruitment of histone deacetylase 2 inhibits interleukin-1beta-induced histone H4 acetylation on lysines 8 and 12.
    Mol Cell Biol. 20: 6891-903.
  8. Kobor, M.S. et al (2004) A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin.
    PLoS Biol. 2: E131.
  9. Lin, X. et al. (2001) Reversal of GSTP1 CpG island hypermethylation and reactivation of pi-class glutathione S-transferase (GSTP1) expression in human prostate cancer cells by treatment with procainamide.
    Cancer Res. 61: 8611-6.
  10. Nie, M. et al. (2003) Transcriptional regulation of cyclooxygenase 2 by bradykinin and interleukin-1beta in human airway smooth muscle cells: involvement of different promoter elements, transcription factors, and histone h4 acetylation.
    Mol Cell Biol. 23: 9233-44.
  11. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
    J Inflamm (Lond). 8: 1.
  12. Torres-Machorro, A.L. et al. (2015) The Set3 Complex Antagonizes the MYST Acetyltransferase Esa1 in the DNA Damage Response.
    Mol Cell Biol. 35 (21): 3714-25.