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Histone H4 Ac5 antibody

Rabbit anti Histone H4 (Ac5)

Product Type
Polyclonal Antibody
Polyclonal IgG
Histone H4 Ac5

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
Ch E IF IP WB 0.1 ml loader
List Price Your Price

Rabbit anti Histone H2 (Ac5) antibody recognizes Histone H4 when acetylated at lysine 5.

Target Species
Synthetic Peptide
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mammals Expected from Sequence
Amphibia Expected from Sequence
Plants Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Ovalbumin-conjugated peptide: NSGRGAcKGGKGLCc.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Chromatin Immunoprecipitation
ELISA 1/1000
Immunofluorescence 1/400
Western Blotting 1/600
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Source Reference

  1. Turner, B.M. et al. (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabeling with site-specific antibodies.
    FEBS Lett. 253 (1-2): 141-5.

References for Histone H4 Ac5 antibody

  1. Turner, B.M. et al. (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei.
    Cell. 69 (2): 375-84.
  2. Belyaev, N. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.
    Hum Genet. 97 (5): 573-8.
  3. Ito, K. et al. (2000) Glucocorticoid receptor recruitment of histone deacetylase 2 inhibits interleukin-1beta-induced histone H4 acetylation on lysines 8 and 12.
    Mol Cell Biol. 20: 6891-903.
  4. Lacoste, N. et al. (2002) Disruptor of telomeric silencing-1 is a chromatin-specific histone H3 methyltransferase.
    J Biol Chem. 277: 30421-4.
  5. Nie, M. et al. (2003) Transcriptional regulation of cyclooxygenase 2 by bradykinin and interleukin-1beta in human airway smooth muscle cells: involvement of different promoter elements, transcription factors, and histone h4 acetylation.
    Mol Cell Biol. 23: 9233-44.
  6. Tanny, J.C. et al. (2004) Budding yeast silencing complexes and regulation of Sir2 activity by protein-protein interactions.
    Mol Cell Biol. 24: 6931-46.
  7. Nie, M. et al. (2005) beta2-Adrenoceptor agonists, like glucocorticoids, repress eotaxin gene transcription by selective inhibition of histone H4 acetylation.
    J Immunol. 175: 478-86.
  8. Spada, F. et al. (2005) Histone H4 post-translational modifications in chordate mitotic and endoreduplicative cell cycles.
    J Cell Biochem. 95: 885-901.
  9. View The Latest Product References
  10. McManus, K.J. et al. (2006) The transcriptional regulator CBP has defined spatial associations within interphase nuclei.
    PLoS Comput Biol. 2: e139.
  11. Auger, A. et al. (2008) Eaf1 is the platform for NuA4 molecular assembly that evolutionarily links chromatin acetylation to ATP-dependent exchange of histone H2A variants.
    Mol Cell Biol. 28: 2257-70.
  12. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
    J Inflamm (Lond). 8 (1): 1.
  13. Krogan, N.T. et al. (2012) APETALA2 negatively regulates multiple floral organ identity genes in Arabidopsis by recruiting the co-repressor TOPLESS and the histone deacetylase HDA19.
    Development. 139: 4180-90.
  14. Havasi, A. et al. (2013) Histone Acetyl Transferase (HAT) HBO1 and JADE1 in Epithelial Cell Regeneration.
    Am J Pathol. 182: 152-62.

Chromatin Immunoprecipitation


Western Blotting

Entrez Gene
GO Terms
GO:0005515 protein binding
GO:0000723 telomere maintenance
GO:0000786 nucleosome
GO:0003677 DNA binding
GO:0005654 nucleoplasm
GO:0048015 phosphatidylinositol-mediated signaling
GO:0034080 CenH3-containing nucleosome assembly at centromere
GO:0045653 negative regulation of megakaryocyte differentiation



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