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Histone H4 Ac5 antibody

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Anti Histone H4 (Ac5) Antibody thumbnail image 1 Anti Histone H4 (Ac5) Antibody thumbnail image 2 Anti Histone H4 (Ac5) Antibody thumbnail image 3 Anti Histone H4 (Ac5) Antibody thumbnail image 4
Anti Histone H4 (Ac5) Antibody gallery image 1
Published customer image:
Rabbit anti Histone H4 (Ac5) antibody (AHP962) used for the detection of acetylated histone H4 in rat colitis tissue lysates by western blotting.
Image caption:
Acetylation on histone 4 (H4) specific lysine residues 5 (K5) (A), 8 (K8) (B), 12 (K12) (C) and 16 (K16) (D) in a Sham (control) and trinitrobenzene sulfonic acid (TNBS) rat model of colitis. Results were obtained by Western blotting. In order to evaluate changes in density from different western blotting experiments control densitometry was denoted as 100% and differences were accounted as increase percentage of the control. Representative examples of bands obtained are also illustrated. Columns represent the densitometric evaluation of three independent experiments (mean ± SEM). (*p <0.05 vs Sham proximal or Sham distal respectively).

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N. Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan 27;8(1):1.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Histone H4 (Ac5) Antibody gallery image 2
Published customer image:
Rabbit anti Histone H4 (Ac5) antibody (AHP962) used for the detection of acetylated histone H4 in Dextran sulphate treated rat tissue lysates by western blotting.
Image caption:
Acetylation on histone 4 (H4) specific lysine residues 5 (K5), 8 (K8), 12 (K12) and 16 (K16) in Lewis and Sprague-Dawley dextran sulfate sodium (5% DSS). A: Representative bands of H4K5, K8, K12 and K16 acetylation. Lanes for Lewis rats represent: non-DSS treated (control) and DSS-treated. Likewise representative bands are illustrated for the Sprague-Dawley rats. Graphical representation of Western blotting data. H4 acetylation of K5 (B), K8 (C), K12 (D) and K16 (E). Columns represent the mean ± SEM (bar) of three independent experiments.

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N. Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan 27;8(1):1.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Histone H4 (Ac5) Antibody gallery image 3
Published customer image:
Rabbit anti Histone H4 (Ac5) antibody (AHP962) used for the detection of acetylated histone H4 in intestinal tissue lysates by western blotting.
Image caption:
Acetylation on histone 4 (H4) and H4 lysine residues in Crohn's disease. Columns represent the mean ± SEM of three independent experiments. Four biopsies were pooled to obtain sufficient protein for one experiment (50 μg of protein) (*p <0.05 vs control). Pan acetylation on H4 in Crohn's disease (A). Acetylation on histone 4 (H4) specific lysine residues 5 (K5) (B), K8 (C), K12 (D), and 16 (E), in non-inflamed, inflamed tissue and Peyer's patches of Crohn's disease patients. Results were obtained by Western blotting. Columns represent the mean ± SEM of three independent experiments. (*p <0.05 vs control, #p <0.005 vs non-inflamed CD). Representative images of the bands obtained are illustrated.

From: Tsaprouni LG, Ito K, Powell JJ, Adcock IM, Punchard N.
Differential patterns of histone acetylation in inflammatory bowel diseases.
J Inflamm (Lond). 2011 Jan 27;8(1):1.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Histone H4 (Ac5) Antibody gallery image 4
Published customer image:
Rabbit anti Histone H4 (Ac5) antibody used for the detection of acetylated histone H5 in 10T1/2 cells by immunofluorescence.
Image caption:
Spatial Relationship between CBP and Specific Histone H3 Post-Translational Modifications The spatial relationship between CBP and a known product of its HAT activity, acetylated K5 (H4) (A), or an unrelated histone H3 modification, trimethylated K4 (H3) (B), was investigated. Representative and deconvolved high-resolution images (100×) of interphase 10T1/2 cells immunofluorescently labeled with anti-CBP and either anti-AcK9 or anti-tMeK4 counterstained with DAPI are presented. The merged image presents a single plane from the 3-D projection where CBP and AcK9 or tMeK4 are shown in green and red, respectively. A region, identified by the white box, is further magnified to show the spatial relationship between CBP and either post-translational histone modification. Scale bars represent 3 μm.

From: McManus KJ, Stephens DA, Adams NM, Islam SA, Freemont PS, Hendzel MJ (2006) The Transcriptional Regulator CBP Has Defined Spatial Associations within Interphase Nuclei.
PLoS Comput Biol 2(10): e139.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Rabbit anti Histone H4 (Ac5)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP962
Datasheet
Ch E IF IP WB
SDS
0.1 ml loader

Rabbit anti Histone H2 (Ac5) antibody recognizes Histone H4 when acetylated at lysine 5.

Product Details

Target Species
Synthetic Peptide
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mammals Expected from Sequence
Drosophila
Amphibia Expected from Sequence
Yeast
Plants Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to acetylated histone H4 were raised by repeated immunisation of rabbits with highly purified antigen.
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Ovalbumin-conjugated peptide: NSGRGAcKGGKGLCc.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P62805
Entrez Gene
HIST2H4B
GO Terms
GO:0005515 protein binding
GO:0000723 telomere maintenance
GO:0000786 nucleosome
GO:0003677 DNA binding
GO:0005654 nucleoplasm
GO:0048015 phosphatidylinositol-mediated signaling
GO:0034080 CenH3-containing nucleosome assembly at centromere
GO:0045653 negative regulation of megakaryocyte differentiation
Regulatory
For research purposes only

Applications of Histone H4 Ac5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Chromatin Immunoprecipitation
ELISA 1/1000
Immunofluorescence 1/400
Immunoprecipitation
Western Blotting 1/600
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rabbit IgG (Fc):Biotin STAR121B E WB 1 mg loader
Goat anti Rabbit IgG (Fc):FITC STAR121F F 1 mg loader
Goat anti Rabbit IgG (Fc):HRP STAR121P E WB 1 mg loader
Goat anti Rabbit IgG (H/L):HRP STAR124P C E WB 1 mg loader
Sheep anti Rabbit IgG:FITC STAR34B C F 1 mg loader
Sheep anti Rabbit IgG:RPE STAR35A F 1 ml loader
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA F IF WB 0.1 mg
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA F IF WB 0.1 mg

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml

Application Based External Images

Chromatin Immunoprecipitation

Immunofluorescence

Western Blotting

Product Specific References

References for Histone H4 Ac5 antibody

  1. Turner, B.M. et al. (1989) Histone H4 acetylation in human cells. Frequency of acetylation at different sites defined by immunolabeling with site-specific antibodies.
    FEBS Lett. 253 (1-2): 141-5.
  2. Turner, B.M. et al. (1992) Histone H4 isoforms acetylated at specific lysine residues define individual chromosomes and chromatin domains in Drosophila polytene nuclei.
    Cell. 69 (2): 375-84.
  3. Belyaev, N. et al. (1996) Differential underacetylation of histones H2A, H3 and H4 on the inactive X chromosome in human female cells.
    Hum Genet. 97 (5): 573-8.
  4. Auger, A. et al. (2008) Eaf1 is the platform for NuA4 molecular assembly that evolutionarily links chromatin acetylation to ATP-dependent exchange of histone H2A variants.
    Mol Cell Biol. 28: 2257-70.
  5. Tsaprouni, L.G. et al. (2011) Differential patterns of histone acetylation in inflammatory bowel diseases.
    J Inflamm (Lond). 8 (1): 1.
  6. Ito, K. et al. (2000) Glucocorticoid receptor recruitment of histone deacetylase 2 inhibits interleukin-1beta-induced histone H4 acetylation on lysines 8 and 12.
    Mol Cell Biol. 20: 6891-903.
  7. Nie, M. et al. (2003) Transcriptional regulation of cyclooxygenase 2 by bradykinin and interleukin-1beta in human airway smooth muscle cells: involvement of different promoter elements, transcription factors, and histone h4 acetylation.
    Mol Cell Biol. 23: 9233-44.
  8. Nie, M. et al. (2005) beta2-Adrenoceptor agonists, like glucocorticoids, repress eotaxin gene transcription by selective inhibition of histone H4 acetylation.
    J Immunol. 175: 478-86.
  9. Tanny, J.C. et al. (2004) Budding yeast silencing complexes and regulation of Sir2 activity by protein-protein interactions.
    Mol Cell Biol. 24: 6931-46.
  10. Spada, F. et al. (2005) Histone H4 post-translational modifications in chordate mitotic and endoreduplicative cell cycles.
    J Cell Biochem. 95: 885-901.
  11. Lacoste, N. et al. (2002) Disruptor of telomeric silencing-1 is a chromatin-specific histone H3 methyltransferase.
    J Biol Chem. 277: 30421-4.
  12. McManus, K.J. et al. (2006) The transcriptional regulator CBP has defined spatial associations within interphase nuclei.
    PLoS Comput Biol. 2: e139.
  13. Krogan, N.T. et al. (2012) APETALA2 negatively regulates multiple floral organ identity genes in Arabidopsis by recruiting the co-repressor TOPLESS and the histone deacetylase HDA19.
    Development. 139: 4180-90.
  14. Havasi, A. et al. (2013) Histone Acetyl Transferase (HAT) HBO1 and JADE1 in Epithelial Cell Regeneration.
    Am J Pathol. 182: 152-62.
  15. Miyamoto, Y. et al. (2015) Involvement of the Tyro3 receptor and its intracellular partner Fyn signaling in Schwann cell myelination.
    Mol Biol Cell. 26 (19): 3489-503.

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