IgG antibody

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Anti Sheep/Goat IgG Antibody thumbnail image 2

Anti Sheep/Goat IgG Antibody thumbnail image 3

Anti Sheep/Goat IgG Antibody thumbnail image 4

Anti Sheep/Goat IgG Antibody gallery image 1 — Detection of Goat anti Human GADD34 (AHP683) in a Western blot analysis of HeLa whole cell lysate using Donkey anti Sheep/Goat IgG:HRP (**STAR88P**).

Anti Sheep/Goat IgG Antibody gallery image 2 — Detection of Sheep anti Human alpha 1 microglobulin in an antigen down ELISA analysis on purified alpha 1 microglobulin using Donkey anti Sheep/Goat IgG:HRP (**STAR88P**).

Anti Sheep/Goat IgG Antibody gallery image 3 — **Published customer image:**
Dylight 488 conjugated donkey anti Sheep IgG antibody used for the visualization of bound sheep anti plasminogen antibody by immunofluorescence.
**Image caption:**
PLG binds to the outer envelope of FT. Laser scanning confocal microscopy of PLG-associated FTLVS was performed as described in "Materials and Methods". Bound huPLG ligand was detected using sheep anti-human PLG antibody followed by incubation with Dylight-488 conjugated donkey, anti-sheep/goat IgG secondary antibody. Samples were visualized using a Zeiss LSM 510 confocal microscope.

From: Clinton SR, Bina JE, Hatch TP, Whitt MA, Miller MA. Binding and activation of host plasminogen on the surface of *Francisella tularensis*.
BMC Microbiol. 2010 Mar 12;10:76.

Anti Sheep/Goat IgG Antibody gallery image 4 — **Published customer image:**
HRP conjugated Donkey anti Sheep/Goat IgG antibody used to detect bound sheep antibody in an ELISA.
**Image caption:**
**Published customer image:**
Horseradish peroxidase conjugated Donkey anti Sheep/Goat IgG antibody used for the measurement of Tci-L4-ES-specific IgG responses in sheep by ELISA.
**Image caption:**
The effects of *T. circumcincta* infection on Tci-L4-ES mediated lymphocyte suppression. Seven helminth-naïve lambs were trickle infected with 2000 *T. circumcincta* L3 three times a week for four weeks. PBMC were harvested at 0, 2, 4 and 6 weeks from the start of infection and cultured for 72 h with PBS alone (PBS), 5 μg/mL Con A (Con A + PBS) or 5 μg/mL Con A + 15 μg/mL Tci-L4-ES (Con A + ES). Proliferation was assessed at 72 h by measurement of [3H] thymidine incorporation. Levels of cytokine present in the cell culture supernatants at 54 h were determined by ELISA. (A) Faecal egg count (FEC) data over the course of the experimental infection. (B) Proliferation of PBMC in response to PBS, Con A + PBS and Con A + ES during experimental *T. circumcincta* infection. (C) Levels of serum Tci-L4-ES-specific IgA and IgG during experimental *T. circumcincta* infection. (D-F) Concentrations of IL-4, IL-10 and IFN-γ in PBMC cultures supernatants. Data represents mean ± SEM. a significant difference between Con A + PBS vs. PBS-stimulated cultures; b significant difference between ConA + ES vs. PBS-stimulated cultures; c significant difference between Con A + PBS vs. Con A + ES stimulated cultures; *P < 0.05, ** P < 0.01, ***P < 0.001 (repeated-measures two-way ANOVA followed by the Bonferroni post hoc test for comparison of means).

From: McNeilly TN, Rocchi M, Bartley Y, Brown JK, Frew D, Longhi C, McLean L, McIntyre J, Nisbet AJ, Wattegedera S, Huntley JF, Matthews JB.
Suppression of ovine lymphocyte activation by *Teladorsagia circumcincta* larval excretory-secretory products.
Vet Res. 2013 Aug 21;44:70..

From: McNeilly, T. *et al.* 2013 Suppression of ovine lymphocyte activation by *Teladorsagia circumcincta* larval excretory-secretory products. Veterinary Research 44:70

Donkey anti Sheep/Goat IgG:Alk. Phos.

Donkey anti Sheep/Goat IgG:HRP

Donkey anti Sheep/Goat IgG:DyLight®488

Donkey anti Sheep/Goat IgG:Dylight®405

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size	Your Price	Quantity
STAR88A Datasheet	C E WB SDS	1 mg	Log in	Get Quote
STAR88P Datasheet	C E P WB SDS	1 ml	Log in	Get Quote
STAR88D488GA Datasheet	F IF SDS	0.1 mg	Log in	Get Quote
STAR88D405GA Datasheet	F IF SDS	0.1 mg	Log in	Get Quote

Donkey anti Sheep/Goat IgG antibody recognizes both sheep and goat IgG, which are immunologically very similar and has been adsorbed against human, rabbit, mouse and rat serum to minimise cross-reactivity.

Product Details

Target Species

Sheep

Species Cross-Reactivity

Target Species	Cross Reactivity
Goat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Alkaline Phosphatase - liquid

Product Form

Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid

Product Form

Purified IgG conjugated to DyLight^®488 - liquid

Product Form

Purified IgG conjugated to DyLight^®405 - liquid

Antiserum Preparation

Antisera to Sheep IgG were raised by repeated immunisation of a donkey with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Buffer Solution

TRIS buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.1%	Sodium Azide
1%	Bovine Serum Albumin
50%	Glycerol

Preservative Stabilisers

0.01% Thiomersal

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Immunogen

Purified IgG from a sheep serum pool.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Storage Information

Storage: Store at +4^oC.

DO NOT FREEZE.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch
Guarantee: 12 months from date of despatch

More Information

Acknowledgements: DyLight^® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Acknowledgements: DyLight^® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
Regulatory: For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/2000	1/5000
Immunohistology - Frozen
Western Blotting
ELISA	1/12800	1/25600
Immunohistology - Frozen	1/50	1/100
Immunohistology - Paraffin
Western Blotting		1/20000
Flow Cytometry	1/50	1/200
Immunofluorescence	1/50	1/200
Flow Cytometry	1/25	1/200
Immunofluorescence

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 50ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 50ul of the suggested working dilution to label 1x10⁶ cells in 100ul

Useful Reagents Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
AbGUARD® HRP Stabilizer Plus	BUF052A	C E P WB	100 ml	Log in	Get Quote
AbGUARD® HRP Stabilizer Plus	BUF052B	C E P WB	500 ml	Log in	Get Quote
AbGUARD® HRP Stabilizer Plus	BUF052C	C E P WB	1000 ml	Log in	Get Quote
TMB Signal+	BUF054A	E	100 ml	Log in	Get Quote
TMB Core	BUF056A	E	100 ml	Log in	Get Quote
TMB Core+	BUF062A	E	100 ml	Log in	Get Quote

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Product Specific References

References for IgG antibody

Singh, M. et al. (1999) A recombinant measles virus expressing hepatitis B virus surface antigen induces humoral immune responses in genetically modified mice.
J Virol. 73 (6): 4823-8.
Tedla, N. et al. (1998) Regulation of T lymphocyte trafficking into lymph nodes during an immune response by the chemokines macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta.
J Immunol. 161 (10): 5663-72.
Turner J et al. (2002) In vivo IL-10 production reactivates chronic pulmonary tuberculosis in C57BL/6 mice.
J Immunol. 169 (11): 6343-51.
Singh, S.K. et al. (2010) Melanin transfer in human skin cells is mediated by filopodia--a model for homotypic and heterotypic lysosome-related organelle transfer.
FASEB J. 24: 3756-69.
Yekta, M.A. et al. (2011) Immunization of sheep with a combination of intiminγ, EspA and EspB decreases Escherichia coli O157:H7 shedding.
Vet Immunol Immunopathol. 140 (1-2): 42-6.
Guitton, C. et al. (2011) Protective cross talk between activated protein C and TNF signaling in vascular endothelial cells: implication of EPCR, noncanonical NF-{kappa}B, and ERK1/2 MAP kinases.
Am J Physiol Cell Physiol. 300: C833-42.
Clinton,. S.R. et al. (2010) Binding and activation of host plasminogen on the surface of Francisella tularensis.
BMC Microbiol. 10: 76.
Chimote, A.A. et al. (2012) Disruption of kv1.3 channel forward vesicular trafficking by hypoxia in human T lymphocytes.
J Biol Chem. 287: 2055-67.
Junqueira-Kipnis, A.P. et al. (2005) Interleukin-10 production by lung macrophages in CBA xid mutant mice infected with Mycobacterium tuberculosis.
Immunology. 115: 246-52.
Nicol, M.Q. et al. (2012) A novel family of peptides with potent activity against influenza A viruses.
J Gen Virol. 93: 980-6.
Singh B et al. (2015) Moraxella catarrhalis Binds Plasminogen To Evade Host Innate Immunity.
Infect Immun. 83 (9): 3458-69.
Al-Jubair, T. et al. (2015) Haemophilus influenzae Type f Hijacks Vitronectin Using Protein H To Resist Host Innate Immunity and Adhere to Pulmonary Epithelial Cells.
J Immunol. 195 (12): 5688-95.
McNeilly, T.N. et al. (2013) Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.
Vet Res. 44: 70.
Garza, J.J. et al. (2017) Serum-mediated Haemonchus contortus larval aggregation differs by larval stage and is enhanced by complement.
Parasite Immunol. 39 (3)
Fager, A.M. et al. (2018) Human platelets express endothelial protein C receptor, which can be utilized to enhance localization of factor VIIa activity.
J Thromb Haemost. 16 (9): 1817-29.
Kim, D.H. et al. (2019) FoxO6-mediated IL-1β induces hepatic insulin resistance and age-related inflammation via the TF/PAR2 pathway in aging and diabetic mice.
Redox Biol. 24: 101184.
Oleaga, A. et al. (2019) Epidemiological surveillance of schistosomiasis outbreak in Corsica (France): Are animal reservoir hosts implicated in local transmission?
PLoS Negl Trop Dis. 13 (6): e0007543.

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