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IgG antibody

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Anti Sheep/Goat IgG Antibody thumbnail image 1 Anti Sheep/Goat IgG Antibody thumbnail image 2 Anti Sheep/Goat IgG Antibody thumbnail image 3 Anti Sheep/Goat IgG Antibody thumbnail image 4
Anti Sheep/Goat IgG Antibody gallery image 1
Detection of Goat anti Human GADD34 (AHP683) in a Western blot analysis of HeLa whole cell lysate using Donkey anti Sheep/Goat IgG:HRP (STAR88P).
Anti Sheep/Goat IgG Antibody gallery image 2
Detection of Sheep anti Human alpha 1 microglobulin in an antigen down ELISA analysis on purified alpha 1 microglobulin using Donkey anti Sheep/Goat IgG:HRP (STAR88P).
Anti Sheep/Goat IgG Antibody gallery image 3
Published customer image:
Dylight 488 conjugated donkey anti Sheep IgG antibody used for the visualization of bound sheep anti plasminogen antibody by immunofluorescence.
Image caption:
PLG binds to the outer envelope of FT. Laser scanning confocal microscopy of PLG-associated FTLVS was performed as described in "Materials and Methods". Bound huPLG ligand was detected using sheep anti-human PLG antibody followed by incubation with Dylight-488 conjugated donkey, anti-sheep/goat IgG secondary antibody. Samples were visualized using a Zeiss LSM 510 confocal microscope.

From: Clinton SR, Bina JE, Hatch TP, Whitt MA, Miller MA. Binding and activation of host plasminogen on the surface of Francisella tularensis.
BMC Microbiol. 2010 Mar 12;10:76.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Sheep/Goat IgG Antibody gallery image 4
Published customer image:
HRP conjugated Donkey anti Sheep/Goat IgG antibody used to detect bound sheep antibody in an ELISA.
Image caption:
Published customer image:
Horseradish peroxidase conjugated Donkey anti Sheep/Goat IgG antibody used for the measurement of Tci-L4-ES-specific IgG responses in sheep by ELISA.
Image caption:
The effects of T. circumcincta infection on Tci-L4-ES mediated lymphocyte suppression. Seven helminth-naïve lambs were trickle infected with 2000 T. circumcincta L3 three times a week for four weeks. PBMC were harvested at 0, 2, 4 and 6 weeks from the start of infection and cultured for 72 h with PBS alone (PBS), 5 μg/mL Con A (Con A + PBS) or 5 μg/mL Con A + 15 μg/mL Tci-L4-ES (Con A + ES). Proliferation was assessed at 72 h by measurement of [3H] thymidine incorporation. Levels of cytokine present in the cell culture supernatants at 54 h were determined by ELISA. (A) Faecal egg count (FEC) data over the course of the experimental infection. (B) Proliferation of PBMC in response to PBS, Con A + PBS and Con A + ES during experimental T. circumcincta infection. (C) Levels of serum Tci-L4-ES-specific IgA and IgG during experimental T. circumcincta infection. (D-F) Concentrations of IL-4, IL-10 and IFN-γ in PBMC cultures supernatants. Data represents mean ± SEM. a significant difference between Con A + PBS vs. PBS-stimulated cultures; b significant difference between ConA + ES vs. PBS-stimulated cultures; c significant difference between Con A + PBS vs. Con A + ES stimulated cultures; *P < 0.05, ** P < 0.01, ***P < 0.001 (repeated-measures two-way ANOVA followed by the Bonferroni post hoc test for comparison of means).

From: McNeilly TN, Rocchi M, Bartley Y, Brown JK, Frew D, Longhi C, McLean L, McIntyre J, Nisbet AJ, Wattegedera S, Huntley JF, Matthews JB.
Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.
Vet Res. 2013 Aug 21;44:70..

From: McNeilly, T. et al. 2013 Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products. Veterinary Research 44:70

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Donkey anti Sheep/Goat IgG:HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

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Product Code Applications Pack Size List Price Your Price Qty
STAR88P
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C E P WB 1 ml loader
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Donkey anti Sheep/Goat IgG antibody recognizes both sheep and goat IgG, which are immunologically very similar and has been adsorbed against human, rabbit, mouse and rat serum to minimise cross-reactivity.

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to sheep IgG were raised by repeated immunisation of donkeys with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Purified IgG from a sheep serum pool.
Approx. Protein Concentrations
1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/12800 1/25600
Immunohistology - Frozen 1/50 1/100
Immunohistology - Paraffin
Western Blotting 1/20000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
TMB Core+ BUF062A E 100 ml
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Description TMB Core+
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
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Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
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Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core

References for IgG antibody

  1. Singh, M. et al. (1999) A recombinant measles virus expressing hepatitis B virus surface antigen induces humoral immune responses in genetically modified mice.
    J Virol. 73 (6): 4823-8.
  2. Tedla, N. et al. (1998) Regulation of T lymphocyte trafficking into lymph nodes during an immune response by the chemokines macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta.
    J Immunol. 161 (10): 5663-72.
  3. Turner J et al. (2002) In vivo IL-10 production reactivates chronic pulmonary tuberculosis in C57BL/6 mice.
    J Immunol. 169 (11): 6343-51.
  4. Singh, S.K. et al. (2010) Melanin transfer in human skin cells is mediated by filopodia--a model for homotypic and heterotypic lysosome-related organelle transfer.
    FASEB J. 24: 3756-69.
  5. Yekta, M.A. et al. (2011) Immunization of sheep with a combination of intiminγ, EspA and EspB decreases Escherichia coli O157:H7 shedding.
    Vet Immunol Immunopathol. 140 (1-2): 42-6.
  6. Guitton, C. et al. (2011) Protective cross talk between activated protein C and TNF signaling in vascular endothelial cells: implication of EPCR, noncanonical NF-{kappa}B, and ERK1/2 MAP kinases.
    Am J Physiol Cell Physiol. 300: C833-42.
  7. Clinton,. S.R. et al. (2010) Binding and activation of host plasminogen on the surface of Francisella tularensis.
    BMC Microbiol. 10: 76.
  8. Chimote, A.A. et al. (2012) Disruption of kv1.3 channel forward vesicular trafficking by hypoxia in human T lymphocytes.
    J Biol Chem. 287: 2055-67.
    9. View The Latest Product References
  9. Junqueira-Kipnis, A.P. et al. (2005) Interleukin-10 production by lung macrophages in CBA xid mutant mice infected with Mycobacterium tuberculosis.
    Immunology. 115: 246-52.
  10. Nicol, M.Q. et al. (2012) A novel family of peptides with potent activity against influenza A viruses.
    J Gen Virol. 93: 980-6.
  11. Singh B et al. (2015) Moraxella catarrhalis Binds Plasminogen To Evade Host Innate Immunity.
    Infect Immun. 83 (9): 3458-69.
  12. Al-Jubair, T. et al. (2015) Haemophilus influenzae Type f Hijacks Vitronectin Using Protein H To Resist Host Innate Immunity and Adhere to Pulmonary Epithelial Cells.
    J Immunol. 195 (12): 5688-95.
  13. McNeilly, T.N. et al. (2013) Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.
    Vet Res. 44: 70.
  14. Garza, J.J. et al. (2017) Serum-mediated Haemonchus contortus larval aggregation differs by larval stage and is enhanced by complement.
    Parasite Immunol. 39 (3)
  15. Fager, A.M. et al. (2018) Human platelets express endothelial protein C receptor, which can be utilized to enhance localization of factor VIIa activity.
    J Thromb Haemost. 16 (9): 1817-29.
  16. Kim, D.H. et al. (2019) FoxO6-mediated IL-1β induces hepatic insulin resistance and age-related inflammation via the TF/PAR2 pathway in aging and diabetic mice.
    Redox Biol. 24: 101184.
  17. Oleaga, A. et al. (2019) Epidemiological surveillance of schistosomiasis outbreak in Corsica (France): Are animal reservoir hosts implicated in local transmission?
    PLoS Negl Trop Dis. 13 (6): e0007543.
  18. Gerlini, G. et al. (2022) Tolerogenic IDO1+CD83- Langerhans Cells in Sentinel Lymph Nodes of Patients with Melanoma.
    Int J Mol Sci. 23 (7): 3441.

Immunofluorescence

Immunohistology - Paraffin

RRID
AB_322719
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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