IgG antibody




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WB E IF ResetDonkey anti Sheep/Goat IgG:DyLight®488
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgG
Donkey anti Sheep/Goat IgG antibody recognizes both sheep and goat IgG, which are immunologically very similar and has been adsorbed against human, rabbit, mouse and rat serum to minimise cross-reactivity. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to DyLight®488 - liquid
- Antiserum Preparation
- Antisera to sheep IgG were raised by repeated immunisation of donkeys with highly purified antigen. Purified IgG was prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Purified IgG from a sheep serum pool.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Dylight®488 493 518 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | ![]() |
1/50 | 1/200 |
Immunofluorescence | ![]() |
1/50 | 1/200 |
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 1x106 cells in 100ul.
References for IgG antibody
-
Singh, M. et al. (1999) A recombinant measles virus expressing hepatitis B virus surface antigen induces humoral immune responses in genetically modified mice.
J Virol. 73 (6): 4823-8. -
Tedla, N. et al. (1998) Regulation of T lymphocyte trafficking into lymph nodes during an immune response by the chemokines macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta.
J Immunol. 161 (10): 5663-72. -
Turner J et al. (2002) In vivo IL-10 production reactivates chronic pulmonary tuberculosis in C57BL/6 mice.
J Immunol. 169 (11): 6343-51. -
Singh, S.K. et al. (2010) Melanin transfer in human skin cells is mediated by filopodia--a model for homotypic and heterotypic lysosome-related organelle transfer.
FASEB J. 24: 3756-69. -
Yekta, M.A. et al. (2011) Immunization of sheep with a combination of intiminγ, EspA and EspB decreases Escherichia coli O157:H7 shedding.
Vet Immunol Immunopathol. 140 (1-2): 42-6. -
Guitton, C. et al. (2011) Protective cross talk between activated protein C and TNF signaling in vascular endothelial cells: implication of EPCR, noncanonical NF-{kappa}B, and ERK1/2 MAP kinases.
Am J Physiol Cell Physiol. 300: C833-42. -
Clinton,. S.R. et al. (2010) Binding and activation of host plasminogen on the surface of Francisella tularensis.
BMC Microbiol. 10: 76. -
Chimote, A.A. et al. (2012) Disruption of kv1.3 channel forward vesicular trafficking by hypoxia in human T lymphocytes.
J Biol Chem. 287: 2055-67.
View The Latest Product References
-
Junqueira-Kipnis, A.P. et al. (2005) Interleukin-10 production by lung macrophages in CBA xid mutant mice infected with Mycobacterium tuberculosis.
Immunology. 115: 246-52. -
Nicol, M.Q. et al. (2012) A novel family of peptides with potent activity against influenza A viruses.
J Gen Virol. 93: 980-6. -
Singh B et al. (2015) Moraxella catarrhalis Binds Plasminogen To Evade Host Innate Immunity.
Infect Immun. 83 (9): 3458-69. -
Al-Jubair, T. et al. (2015) Haemophilus influenzae Type f Hijacks Vitronectin Using Protein H To Resist Host Innate Immunity and Adhere to Pulmonary Epithelial Cells.
J Immunol. 195 (12): 5688-95. -
McNeilly, T.N. et al. (2013) Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.
Vet Res. 44: 70. -
Garza, J.J. et al. (2017) Serum-mediated Haemonchus contortus larval aggregation differs by larval stage and is enhanced by complement.
Parasite Immunol. 39 (3) -
Fager, A.M. et al. (2018) Human platelets express endothelial protein C receptor, which can be utilized to enhance localization of factor VIIa activity.
J Thromb Haemost. 16 (9): 1817-29. -
Kim, D.H. et al. (2019) FoxO6-mediated IL-1β induces hepatic insulin resistance and age-related inflammation via the TF/PAR2 pathway in aging and diabetic mice.
Redox Biol. 24: 101184. -
Oleaga, A. et al. (2019) Epidemiological surveillance of schistosomiasis outbreak in Corsica (France): Are animal reservoir hosts implicated in local transmission?
PLoS Negl Trop Dis. 13 (6): e0007543. -
Gerlini, G. et al. (2022) Tolerogenic IDO1+CD83- Langerhans Cells in Sentinel Lymph Nodes of Patients with Melanoma.
Int J Mol Sci. 23 (7): 3441. -
Richter, C. et al. (2016) Moonlighting of Helicobacter pylori catalase protects against complement-mediated killing by utilising the host molecule vitronectin.
Sci Rep. 6: 24391. -
Rojas, J.M. et al. (2022) Adenoviral delivery of soluble ovine OX40L or CD70 costimulatory molecules improves adaptive immune responses to a model antigen in sheep.
Front Cell Infect Microbiol. 12: 1010873.
- RRID
- AB_10848346
STAR88D488GA



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