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IgG antibody

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Anti Sheep/Goat IgG Antibody thumbnail image 1 Anti Sheep/Goat IgG Antibody thumbnail image 2 Anti Sheep/Goat IgG Antibody thumbnail image 3 Anti Sheep/Goat IgG Antibody thumbnail image 4
Anti Sheep/Goat IgG Antibody gallery image 1
Detection of Goat anti Human GADD34 (AHP683) in a Western blot analysis of HeLa whole cell lysate using Donkey anti Sheep/Goat IgG:HRP (STAR88P).
Anti Sheep/Goat IgG Antibody gallery image 2
Detection of Sheep anti Human alpha 1 microglobulin (AHP2149) in an antigen down ELISA analysis on purified alpha 1 microglobulin using Donkey anti Sheep/Goat IgG:HRP (STAR88P).
Anti Sheep/Goat IgG Antibody gallery image 3
Published customer image:
Dylight 488 conjugated donkey anti Sheep IgG antibody used for the visualization of bound sheep anti plasminogen antibody by immunofluorescence.
Image caption:
PLG binds to the outer envelope of FT. Laser scanning confocal microscopy of PLG-associated FTLVS was performed as described in "Materials and Methods". Bound huPLG ligand was detected using sheep anti-human PLG antibody followed by incubation with Dylight-488 conjugated donkey, anti-sheep/goat IgG secondary antibody. Samples were visualized using a Zeiss LSM 510 confocal microscope.

From: Clinton SR, Bina JE, Hatch TP, Whitt MA, Miller MA. Binding and activation of host plasminogen on the surface of Francisella tularensis.
BMC Microbiol. 2010 Mar 12;10:76.
Anti Sheep/Goat IgG Antibody gallery image 4
Published customer image:
HRP conjugated Donkey anti Sheep/Goat IgG antibody used to detect bound sheep antibody in an ELISA.
Image caption:
Published customer image:
Horseradish peroxidase conjugated Donkey anti Sheep/Goat IgG antibody used for the measurement of Tci-L4-ES-specific IgG responses in sheep by ELISA.
Image caption:
The effects of T. circumcincta infection on Tci-L4-ES mediated lymphocyte suppression. Seven helminth-naïve lambs were trickle infected with 2000 T. circumcincta L3 three times a week for four weeks. PBMC were harvested at 0, 2, 4 and 6 weeks from the start of infection and cultured for 72 h with PBS alone (PBS), 5 μg/mL Con A (Con A + PBS) or 5 μg/mL Con A + 15 μg/mL Tci-L4-ES (Con A + ES). Proliferation was assessed at 72 h by measurement of [3H] thymidine incorporation. Levels of cytokine present in the cell culture supernatants at 54 h were determined by ELISA. (A) Faecal egg count (FEC) data over the course of the experimental infection. (B) Proliferation of PBMC in response to PBS, Con A + PBS and Con A + ES during experimental T. circumcincta infection. (C) Levels of serum Tci-L4-ES-specific IgA and IgG during experimental T. circumcincta infection. (D-F) Concentrations of IL-4, IL-10 and IFN-γ in PBMC cultures supernatants. Data represents mean ± SEM. a significant difference between Con A + PBS vs. PBS-stimulated cultures; b significant difference between ConA + ES vs. PBS-stimulated cultures; c significant difference between Con A + PBS vs. Con A + ES stimulated cultures; *P < 0.05, ** P < 0.01, ***P < 0.001 (repeated-measures two-way ANOVA followed by the Bonferroni post hoc test for comparison of means).

From: McNeilly TN, Rocchi M, Bartley Y, Brown JK, Frew D, Longhi C, McLean L, McIntyre J, Nisbet AJ, Wattegedera S, Huntley JF, Matthews JB.
Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.
Vet Res. 2013 Aug 21;44:70..

From: McNeilly, T. et al. 2013 Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products. Veterinary Research 44:70

Donkey anti Sheep/Goat IgG:Alk. Phos.

Donkey anti Sheep/Goat IgG:HRP

Donkey anti Sheep/Goat IgG:DyLight®488

Donkey anti Sheep/Goat IgG:Dylight®405

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
STAR88A
Datasheet
C E WB
SDS
1 mg loader

STAR88P
Datasheet
C E P WB
SDS
1 ml loader

STAR88D488GA
Datasheet
F IF
SDS
0.1 mg loader

STAR88D405GA
Datasheet
F IF
SDS
0.1 mg loader

Donkey anti Sheep/Goat IgG antibody recognizes both sheep and goat IgG, which are immunologically very similar and has been adsorbed against human, rabbit, mouse and rat serum to minimise cross-reactivity.

Product Details

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Purified IgG conjugated to DyLight®488 - liquid
Product Form
Purified IgG conjugated to DyLight®405 - liquid
Antiserum Preparation
Antisera to Sheep IgG were raised by repeated immunisation of a donkey with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
TRIS buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
1%Bovine Serum Albumin
50%Glycerol
Preservative Stabilisers
0.01% Thiomersal
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Purified IgG from a sheep serum pool.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Acknowledgements
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/5000
Immunohistology - Frozen
Western Blotting
ELISA 1/12800 1/25600
Immunohistology - Frozen 1/50 1/100
Immunohistology - Paraffin
Western Blotting 1/20000
Flow Cytometry 1/50 1/200
Immunofluorescence 1/50 1/200
Flow Cytometry 1/25 1/200
Immunofluorescence
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 50ul of the suggested working dilution to label 1x106 cells in 100ul
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Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
TMB Signal+ BUF054A E 100 ml
TMB Core BUF056A E 100 ml
TMB Core+ BUF062A E 100 ml
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml

Application Based External Images

Immunofluorescence

Immunohistology - Paraffin

Product Specific References

References for IgG antibody

  1. Singh, M. et al. (1999) A recombinant measles virus expressing hepatitis B virus surface antigen induces humoral immune responses in genetically modified mice.
    J Virol. 73 (6): 4823-8.
  2. Tedla, N. et al. (1998) Regulation of T lymphocyte trafficking into lymph nodes during an immune response by the chemokines macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta.
    J Immunol. 161 (10): 5663-72.
  3. Turner J et al. (2002) In vivo IL-10 production reactivates chronic pulmonary tuberculosis in C57BL/6 mice.
    J Immunol. 169 (11): 6343-51.
  4. Singh, S.K. et al. (2010) Melanin transfer in human skin cells is mediated by filopodia--a model for homotypic and heterotypic lysosome-related organelle transfer.
    FASEB J. 24: 3756-69.
  5. Yekta, M.A. et al. (2011) Immunization of sheep with a combination of intiminγ, EspA and EspB decreases Escherichia coli O157:H7 shedding.
    Vet Immunol Immunopathol. 140 (1-2): 42-6.
  6. Guitton, C. et al. (2011) Protective cross talk between activated protein C and TNF signaling in vascular endothelial cells: implication of EPCR, noncanonical NF-{kappa}B, and ERK1/2 MAP kinases.
    Am J Physiol Cell Physiol. 300: C833-42.
  7. Clinton,. S.R. et al. (2010) Binding and activation of host plasminogen on the surface of Francisella tularensis.
    BMC Microbiol. 10: 76.
  8. Chimote, A.A. et al. (2012) Disruption of kv1.3 channel forward vesicular trafficking by hypoxia in human T lymphocytes.
    J Biol Chem. 287: 2055-67.
  9. Junqueira-Kipnis, A.P. et al. (2005) Interleukin-10 production by lung macrophages in CBA xid mutant mice infected with Mycobacterium tuberculosis.
    Immunology. 115: 246-52.
  10. Nicol, M.Q. et al. (2012) A novel family of peptides with potent activity against influenza A viruses.
    J Gen Virol. 93: 980-6.
  11. Singh B et al. (2015) Moraxella catarrhalis Binds Plasminogen To Evade Host Innate Immunity.
    Infect Immun. 83 (9): 3458-69.
  12. Al-Jubair, T. et al. (2015) Haemophilus influenzae Type f Hijacks Vitronectin Using Protein H To Resist Host Innate Immunity and Adhere to Pulmonary Epithelial Cells.
    J Immunol. 195 (12): 5688-95.
  13. McNeilly, T.N. et al. (2013) Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.
    Vet Res. 44: 70.
  14. Garza, J.J. et al. (2017) Serum-mediated Haemonchus contortus larval aggregation differs by larval stage and is enhanced by complement.
    Parasite Immunol. 39 (3)

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