IgG antibody




Donkey anti Sheep/Goat IgG:Alk. Phos.
Donkey anti Sheep/Goat IgG:FITC
Donkey anti Sheep/Goat IgG:HRP
Donkey anti Sheep/Goat IgG:DyLight®488
Donkey anti Sheep/Goat IgG:Dylight®405
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
STAR88A | C E WB | 1 mg |
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STAR88F | C | 1 mg |
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STAR88P | C E P WB | 1 ml |
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STAR88D488GA | F IF | 0.1 mg |
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STAR88D405GA | F IF | 0.1 mg |
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Product Details
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Product Form
- Purified IgG conjugated to DyLight®488 - liquid
- Product Form
- Purified IgG conjugated to DyLight®405 - liquid
- Antiserum Preparation
- Antisera to Sheep IgG were raised by repeated immunisation of a donkey with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.1% Sodium Azide 1% Bovine Serum Albumin 50% Glycerol - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- 0.01% Thiomersal
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Purified IgG from a sheep serum pool.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use - Guarantee
- 18 months from date of despatch.
- Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- 12 months from date of despatch.
- Guarantee
- 18 months from date of despatch
- Guarantee
- 18 months from date of despatch
More Information
- Acknowledgements
- DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
- Acknowledgements
- DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries
- Regulatory
- For research purposes only
Applications of IgG antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/2000 | 1/5000 | |
Immunohistology - Frozen | |||
Western Blotting | |||
Immunohistology - Frozen | 1/100 | 1/200 | |
ELISA | 1/12800 | 1/25600 | |
Immunohistology - Frozen | 1/50 | 1/100 | |
Immunohistology - Paraffin | |||
Western Blotting | 1/20000 | ||
Flow Cytometry | 1/50 | 1/200 | |
Immunofluorescence | 1/50 | 1/200 | |
Flow Cytometry | 1/25 | 1/200 | |
Immunofluorescence |
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 1x106 cells in 100ul
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
AbGUARD® HRP Stabilizer Plus | BUF052A | C E P WB | 100 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052B | C E P WB | 500 ml | ||
AbGUARD® HRP Stabilizer Plus | BUF052C | C E P WB | 1000 ml | ||
TMB Signal+ | BUF054A | E | 100 ml | ||
TMB Core | BUF056A | E | 100 ml | ||
TMB Core+ | BUF062A | E | 100 ml |
Product Specific References
References for IgG antibody
-
Singh, M. et al. (1999) A recombinant measles virus expressing hepatitis B virus surface antigen induces humoral immune responses in genetically modified mice.
J Virol. 73 (6): 4823-8. -
Tedla, N. et al. (1998) Regulation of T lymphocyte trafficking into lymph nodes during an immune response by the chemokines macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta.
J Immunol. 161 (10): 5663-72. -
Turner J et al. (2002) In vivo IL-10 production reactivates chronic pulmonary tuberculosis in C57BL/6 mice.
J Immunol. 169 (11): 6343-51. -
Singh, S.K. et al. (2010) Melanin transfer in human skin cells is mediated by filopodia--a model for homotypic and heterotypic lysosome-related organelle transfer.
FASEB J. 24: 3756-69. -
Yekta, M.A. et al. (2011) Immunization of sheep with a combination of intiminγ, EspA and EspB decreases Escherichia coli O157:H7 shedding.
Vet Immunol Immunopathol. 140 (1-2): 42-6. -
Guitton, C. et al. (2011) Protective cross talk between activated protein C and TNF signaling in vascular endothelial cells: implication of EPCR, noncanonical NF-{kappa}B, and ERK1/2 MAP kinases.
Am J Physiol Cell Physiol. 300: C833-42. -
Clinton,. S.R. et al. (2010) Binding and activation of host plasminogen on the surface of Francisella tularensis.
BMC Microbiol. 10: 76. -
Chimote, A.A. et al. (2012) Disruption of kv1.3 channel forward vesicular trafficking by hypoxia in human T lymphocytes.
J Biol Chem. 287: 2055-67. -
Junqueira-Kipnis, A.P. et al. (2005) Interleukin-10 production by lung macrophages in CBA xid mutant mice infected with Mycobacterium tuberculosis.
Immunology. 115: 246-52. -
Nicol, M.Q. et al. (2012) A novel family of peptides with potent activity against influenza A viruses.
J Gen Virol. 93: 980-6. -
Singh B et al. (2015) Moraxella catarrhalis Binds Plasminogen To Evade Host Innate Immunity.
Infect Immun. 83 (9): 3458-69. -
Al-Jubair, T. et al. (2015) Haemophilus influenzae Type f Hijacks Vitronectin Using Protein H To Resist Host Innate Immunity and Adhere to Pulmonary Epithelial Cells.
J Immunol. 195 (12): 5688-95. -
McNeilly, T.N. et al. (2013) Suppression of ovine lymphocyte activation by Teladorsagia circumcincta larval excretory-secretory products.
Vet Res. 44: 70. -
Garza, J.J. et al. (2017) Serum-mediated Haemonchus contortus larval aggregation differs by larval stage and is enhanced by complement.
Parasite Immunol. 39 (3)