14-3-3 Sigma antibody

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Rabbit anti 14-3-3 Sigma

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1050 WB 0.1 ml
Rabbit anti 14-3-3σ antibody recognizes 14-3-3 sigma in all mammals. It is a member of the 14-3-3 family which consists of 30 kDa proteins involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isotypes and are regulated by phosphorylation of both 14-3-3 and the bound protein.

14-3-3σ is expressed mostly in epithelial cells and has been associated with cancers including breast, nasopharyngeal, endometrial adenocarcinoma, prostate, epithelial ovarian, colorectal, adenoid cystic, and papillary.

14-3-3σ binds c-fos activating the MAPK pathway. Sequestration of Bax by 14-3-3σ inhibits apoptosis whereas sequestration of 14-3-3 sigma by CARP-1 induces it. Mitosis is regulated by 14-3-3σ through binding keratin filaments and sequestering cyclin B1 and cdc2. p73-dependent induction of 14-3-3 sigma regulates chemo sensitivity in breast cancers with p53 mutations. In reaction to DNA damage and along with RACK1, 14-3-3σ targets, DeltaNp63alpha for proteasome degradation. 14-3-3 also negatively regulates both the glucocorticoid receptor and the activity of PKB/Akt which stabilizes p27 Kip1.

Rabbit anti 14-3-3σ antibody may not react with recombinant proteins that are not N-acetylated.

Product Details

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Bovine
Chicken
Rat
Mouse
Rabbit
Mammals Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Serum - liquid
Antiserum Preparation
Antisera to sheep 14-3-3 sigma were raised by repeated immunisations of rabbit with highly purified antigen.
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 sigma.

Peptide sequence: Ac.MERASLIQKAC

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
O77642 Related reagents
Entrez Gene
SFN Related reagents
GO Terms
GO:0005576 extracellular region
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0019904 protein domain specific binding
Regulatory
For research purposes only

Applications of 14-3-3 Sigma antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/3000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1050 detects a band of approximately 30kDa in HEK293 cell lysates.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti 14-3-3 Isoform Panel PAN017 25 µl X 7 E* P* WB*
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*

Product Specific References

References for 14-3-3 Sigma antibody

  1. Taurino, F. et al. (2014) Short-term type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver.
    Eur J Clin Invest. 44 (4): 350-8.
  2. De, S. et al. (2012) Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development
    BMC Res Notes. 5: 57.

Further Reading

  1. Aitken, A. (2006) 14-3-3 proteins: a historic overview.
    Semin Cancer Biol. 16 (3): 162-72.